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2oxb

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{{STRUCTURE_2oxb| PDB=2oxb | SCENE= }}
{{STRUCTURE_2oxb| PDB=2oxb | SCENE= }}
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'''Crystal structure of a cell-wall invertase (E203Q) from Arabidopsis thaliana in complex with sucrose'''
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===Crystal structure of a cell-wall invertase (E203Q) from Arabidopsis thaliana in complex with sucrose===
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==Overview==
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In the present study, we report on the X-ray crystallographic structure of a GH32 invertase mutant, (i.e., the Arabidopsis thaliana cell-wall invertase 1-E203Q, AtcwINV1-mutant) in complex with sucrose. This structure was solved to reveal the features of sugar binding in the catalytic pocket. However, as demonstrated by the X-ray structure the sugar binding and the catalytic pocket arrangement is significantly altered as compared with what was expected based on previous X-ray structures on GH-J clan enzymes. We performed a series of docking and molecular dynamics simulations on various derivatives of AtcwINV1 to reveal the reasons behind this modified sugar binding. Our results demonstrate that the E203Q mutation introduced into the catalytic pocket triggers conformational changes that alter the wild type substrate binding. In addition, this study also reveals the putative productive sucrose binding modus in the wild type enzyme. Proteins 2007. (c) 2007 Wiley-Liss, Inc.
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(as it appears on PubMed at http://www.pubmed.gov), where 17963237 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17963237}}
==About this Structure==
==About this Structure==
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[[Category: Roy, K Le.]]
[[Category: Roy, K Le.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:51:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:04:14 2008''

Revision as of 09:04, 28 July 2008

Image:2oxb.png

Template:STRUCTURE 2oxb

Crystal structure of a cell-wall invertase (E203Q) from Arabidopsis thaliana in complex with sucrose

Template:ABSTRACT PUBMED 17963237

About this Structure

2OXB is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

An alternate sucrose binding mode in the E203Q Arabidopsis invertase mutant: An X-ray crystallography and docking study., Matrai J, Lammens W, Jonckheer A, Le Roy K, Rabijns A, Van den Ende W, De Maeyer M, Proteins. 2007 Oct 26;. PMID:17963237

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