2v03

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{{STRUCTURE_2v03| PDB=2v03 | SCENE= }}
{{STRUCTURE_2v03| PDB=2v03 | SCENE= }}
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'''HIGH RESOLUTION STRUCTURE AND CATALYSIS OF AN O-ACETYLSERINE SULFHYDRYLASE'''
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===HIGH RESOLUTION STRUCTURE AND CATALYSIS OF AN O-ACETYLSERINE SULFHYDRYLASE===
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==Overview==
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The crystal structure of the dimeric O-acetylserine sulfhydrylase isozyme B from Escherichia coli (CysM), complexed with the substrate analog citrate, has been determined at 1.33 A resolution by X-ray diffraction analysis. The C1-carboxylate of citrate was bound at the carboxylate position of O-acetylserine, whereas the C6-carboxylate adopted two conformations. The activity of the enzyme and of several active center mutants was determined using an assay based on O-acetylserine and thio-nitrobenzoate (TNB). The unnatural substrate TNB was modeled into the reported structure. The substrate model and the observed mutant activities may facilitate future protein engineering attempts designed to broaden the substrate spectrum of the enzyme. A comparison of the reported structure with previously published CysM structures revealed large conformational changes. One of the crystal forms contained two dimers, each of which comprised one subunit in a closed and one in an open conformation. Although the homodimer asymmetry was most probably caused by crystal packing, it indicates that the enzyme can adopt such a state in solution, which may be relevant for the catalytic reaction.
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{{ABSTRACT_PUBMED_17894825}}
==About this Structure==
==About this Structure==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: X-ray diffraction]]
[[Category: X-ray diffraction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:56:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:06:08 2008''

Revision as of 09:06, 28 July 2008

Image:2v03.png

Template:STRUCTURE 2v03

HIGH RESOLUTION STRUCTURE AND CATALYSIS OF AN O-ACETYLSERINE SULFHYDRYLASE

Template:ABSTRACT PUBMED 17894825

About this Structure

2V03 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli., Zocher G, Wiesand U, Schulz GE, FEBS J. 2007 Oct;274(20):5382-9. Epub 2007 Sep 26. PMID:17894825

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