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| - | [[Image:1sm7.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1sm7| PDB=1sm7 | SCENE= }} | | {{STRUCTURE_1sm7| PDB=1sm7 | SCENE= }} |
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| - | '''Solution structure of the recombinant pronapin precursor, BnIb.'''
| + | ===Solution structure of the recombinant pronapin precursor, BnIb.=== |
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| - | ==Overview==
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| - | NMR spectroscopy has been used to determine the solution structure of the precursor form of the recombinant napin BnIb, rproBnIb, a 2S albumin, 109-residue protein from the seeds of Brassica napus. More than 90% of the side-chain proton resonances were unambiguously assigned from the analysis of two-dimensional correlation (COSY), total correlation (TOCSY), and nuclear Overhauser effect (NOESY) spectra. The final structures were computed by using restrained molecular dynamics on the basis of 1316 upper-limit distance constraints derived from NOE cross-correlation intensities. The computed structures exhibited a root-mean-square deviation (RMSD) radius of 0.66 A for the backbone and 1.16 A for the side-chain heavy atoms of the structural core. The resulting structure consists of five amphipathic helices arranged in a right-handed super helix, a folding motif found in other proteins of the prolamin superfamily. As in the case of the mature protein, the recombinant precursor behaves as a plant food allergen. To trace out the origin and characteristics of its allergenic properties, rproBnIb was assayed against simulated gastric fluid and found to be very resistant to proteolysis. Also, heat treatment of the protein followed up to 85 degrees C by circular dichroism showed a very limited unfolding, which was recovered after cooling to 20 degrees C, indicating a high thermal stability. These results suggest that rproBnIb, as other 2S albumins, may be able to reach the gut immune system intact. A comparison of the putative epitopes against IgE antibodies of the three members of the prolamine family [2S albumins, nonspecific lipid transfer proteins (nsLTPs), and alpha-amylase/trypsin inhibitors] indicates that there are not common surfaces of interaction with IgE. Though the epitopes appear to be located in different regions of the proteins, they do comply with the requirements of being solvent-exposed and flexible.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15609998}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15609998 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15609998}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1SM7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brassica_napus Brassica napus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SM7 OCA]. | + | 1SM7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brassica_napus Brassica napus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SM7 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: All alpha-helix]] | | [[Category: All alpha-helix]] |
| | [[Category: Right-handed superhelix]] | | [[Category: Right-handed superhelix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:52:31 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:12:14 2008'' |
Revision as of 09:12, 28 July 2008
Template:STRUCTURE 1sm7
Solution structure of the recombinant pronapin precursor, BnIb.
Template:ABSTRACT PUBMED 15609998
About this Structure
1SM7 is a Single protein structure of sequence from Brassica napus. Full experimental information is available from OCA.
Reference
Solution structure and stability against digestion of rproBnIb, a recombinant 2S albumin from rapeseed: relationship to its allergenic properties., Pantoja-Uceda D, Palomares O, Bruix M, Villalba M, Rodriguez R, Rico M, Santoro J, Biochemistry. 2004 Dec 28;43(51):16036-45. PMID:15609998
Page seeded by OCA on Mon Jul 28 12:12:14 2008
