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2hvj

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{{STRUCTURE_2hvj| PDB=2hvj | SCENE= }}
{{STRUCTURE_2hvj| PDB=2hvj | SCENE= }}
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'''Crystal structure of KcsA-Fab-TBA complex in low K+'''
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===Crystal structure of KcsA-Fab-TBA complex in low K+===
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==Overview==
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K(+) channels play essential roles in regulating membrane excitability of many diverse cell types by selectively conducting K(+) ions through their pores. Many diverse molecules can plug the pore and modulate the K(+) current. Quaternary ammonium (QA) ions are a class of pore blockers that have been used for decades by biophysicists to probe the pore, leading to important insights into the structure-function relation of K(+) channels. However, many key aspects of the QA-blocking mechanisms remain unclear to date, and understanding these questions requires high resolution structural information. Here, we address the question of whether intracellular QA blockade causes conformational changes of the K(+) channel selectivity filter. We have solved the structures of the KcsA K(+) channel in complex with tetrabutylammonium (TBA) and tetrabutylantimony (TBSb) under various ionic conditions. Our results demonstrate that binding of TBA or TBSb causes no significant change in the KcsA structure at high concentrations of permeant ions. We did observe the expected conformational change of the filter at low concentration of K(+), but this change appears to be independent of TBA or TBSb blockade.
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{{ABSTRACT_PUBMED_17196615}}
==About this Structure==
==About this Structure==
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[[Category: Potassium channel]]
[[Category: Potassium channel]]
[[Category: Tetrabutylammonium]]
[[Category: Tetrabutylammonium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:45:52 2008''
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Revision as of 09:18, 28 July 2008

Image:2hvj.png

Template:STRUCTURE 2hvj

Crystal structure of KcsA-Fab-TBA complex in low K+

Template:ABSTRACT PUBMED 17196615

About this Structure

2HVJ is a Single protein structure of sequence from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA.

Reference

Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel., Yohannan S, Hu Y, Zhou Y, J Mol Biol. 2007 Feb 23;366(3):806-14. Epub 2006 Dec 2. PMID:17196615

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