1moj

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spinqualitylabelsall models 1moj, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of an archaeal dps-homologue from Halobacterium salinarum

Overview

The crystal structure of the Dps-like (Dps, DNA-protecting protein during, starvation) ferritin protein DpsA from the halophile Halobacterium, salinarum was determined with low endogenous iron content at 1.6-A, resolution. The mechanism of iron uptake and storage was analyzed in this, noncanonical ferritin by three high-resolution structures at successively, increasing iron contents. In the high-iron state of the DpsA protein, up, to 110 iron atoms were localized in the dodecameric protein complex. For, ultimate iron storage, the archaeal ferritin shell comprises iron-binding, sites for iron translocation, oxidation, and nucleation. Initial, iron-protein interactions occur through acidic residues exposed along the, outer surface in proximity to the iron entry pore. This narrow pore, permits translocation of ions toward the ferroxidase centers via two, discrete steps. Iron oxidation proceeds by transient formation of tri-iron, ferroxidase centers. Iron storage by biomineralization inside the ferritin, shell occurs at two iron nucleation centers. Here, a single iron atom, provides a structural seed for iron-oxide cluster formation. The clusters, with up to five iron atoms adopt a geometry that is different from natural, biominerals like magnetite but resembles iron clusters so far known only, from bioinorganic model compounds.

About this Structure

1MOJ is a Single protein structure of sequence from Halobacterium salinarum with FE, MG and NA as ligands. Full crystallographic information is available from OCA.

Reference

Iron-oxo clusters biomineralizing on protein surfaces: structural analysis of Halobacterium salinarum DpsA in its low- and high-iron states., Zeth K, Offermann S, Essen LO, Oesterhelt D, Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13780-5. Epub 2004 Sep 13. PMID:15365182

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