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2ux8

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[[Image:2ux8.gif|left|200px]]
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{{STRUCTURE_2ux8| PDB=2ux8 | SCENE= }}
{{STRUCTURE_2ux8| PDB=2ux8 | SCENE= }}
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'''CRYSTAL STRUCTURE OF SPHINGOMONAS ELODEA ATCC 31461 GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE IN COMPLEX WITH GLUCOSE-1-PHOSPHATE.'''
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===CRYSTAL STRUCTURE OF SPHINGOMONAS ELODEA ATCC 31461 GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE IN COMPLEX WITH GLUCOSE-1-PHOSPHATE.===
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==Overview==
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Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the reversible conversion of glucose-1-phosphate and UTP into UDP-glucose and pyrophosphate, which is a key step in the biosynthetic pathway of gellan gums. Here we present the X-ray crystal structure of the glucose-1-phosphate uridylyltransferase from S. elodea. The S. elodea enzyme shares strong monomeric similarity with glucose-1-phosphate thymidylyltransferase, several structures of which are known, although the quaternary structures of the active enzymes are rather different. A detailed comparison between S. elodea glucose-1-phosphate uridylyltransferase and available thymidylyltransferases is described and shows remarkable structural similarities, despite the low sequence identities between the two divergent groups of proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 17434970 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17434970}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members., Aragao D, Fialho AM, Marques AR, Mitchell EP, Sa-Correia I, Frazao C, J Bacteriol. 2007 Jun;189(12):4520-8. Epub 2007 Apr 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17434970 17434970]
The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members., Aragao D, Fialho AM, Marques AR, Mitchell EP, Sa-Correia I, Frazao C, J Bacteriol. 2007 Jun;189(12):4520-8. Epub 2007 Apr 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17434970 17434970]
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Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate., Aragao D, Marques AR, Frazao C, Enguita FJ, Carrondo MA, Fialho AM, Sa-Correia I, Mitchell EP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):930-4. Epub 2006 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16946483 16946483]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Sphingomonas elodea]]
[[Category: Sphingomonas elodea]]
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Ugpg]]
[[Category: Ugpg]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:43:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:27:38 2008''

Revision as of 09:27, 28 July 2008

Image:2ux8.png

Template:STRUCTURE 2ux8

CRYSTAL STRUCTURE OF SPHINGOMONAS ELODEA ATCC 31461 GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE IN COMPLEX WITH GLUCOSE-1-PHOSPHATE.

Template:ABSTRACT PUBMED 17434970

About this Structure

2UX8 is a Single protein structure of sequence from Sphingomonas elodea. Full crystallographic information is available from OCA.

Reference

The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members., Aragao D, Fialho AM, Marques AR, Mitchell EP, Sa-Correia I, Frazao C, J Bacteriol. 2007 Jun;189(12):4520-8. Epub 2007 Apr 13. PMID:17434970

Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate., Aragao D, Marques AR, Frazao C, Enguita FJ, Carrondo MA, Fialho AM, Sa-Correia I, Mitchell EP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):930-4. Epub 2006 Aug 26. PMID:16946483

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