2nvh

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{{STRUCTURE_2nvh| PDB=2nvh | SCENE= }}
{{STRUCTURE_2nvh| PDB=2nvh | SCENE= }}
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'''Determination of Solvent Content in Cavities in Interleukin-1 Using Experimentally-Phased Electron Density'''
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===Determination of Solvent Content in Cavities in Interleukin-1 Using Experimentally-Phased Electron Density===
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==Overview==
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The extent to which water is present within apolar cavities in proteins remains unclear. In the case of interleukin-1beta (IL-1beta), four independent structures solved by x-ray crystallography indicate that water is not present in the central apolar cavity. In contrast, results from NMR spectroscopy suggest that water has high occupancy within the cavity but is positionally disordered, making it undetectable by standard crystallographic methods. A theoretically based crystallographic-phase refinement technique also suggested that there was the equivalent of two fully occupied water molecules within the apolar cavity. To resolve these discrepancies we sought to obtain an experimentally phased electron density map that was free of possible bias caused by mathematical modeling of the protein or the solvent. By combining native diffraction data with multiple wavelength anomalous data from a platinum derivative, accurate phases were obtained. Using these experimental phases, we estimate that occupancy of the apolar cavity in IL-1beta by solvent is close or equal to zero. Polar cavities in the protein that contain ordered solvent molecules serve as internal controls.
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(as it appears on PubMed at http://www.pubmed.gov), where 17179045 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17179045}}
==About this Structure==
==About this Structure==
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[[Category: Protein folding]]
[[Category: Protein folding]]
[[Category: Water]]
[[Category: Water]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:57:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:28:14 2008''

Revision as of 09:28, 28 July 2008

Image:2nvh.png

Template:STRUCTURE 2nvh

Determination of Solvent Content in Cavities in Interleukin-1 Using Experimentally-Phased Electron Density

Template:ABSTRACT PUBMED 17179045

About this Structure

2NVH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Determination of solvent content in cavities in IL-1beta using experimentally phased electron density., Quillin ML, Wingfield PT, Matthews BW, Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19749-53. Epub 2006 Dec 18. PMID:17179045

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