2dgj

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{{STRUCTURE_2dgj| PDB=2dgj | SCENE= }}
{{STRUCTURE_2dgj| PDB=2dgj | SCENE= }}
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'''Crystal structure of EbhA (756-1003 domain) from Staphylococcus aureus'''
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===Crystal structure of EbhA (756-1003 domain) from Staphylococcus aureus===
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==Overview==
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The 1.1 MDa cell-wall-associated adhesion protein of staphylococci, Ebh, consists of several distinct regions, including a large central region with 52 imperfect repeats of 126 amino acid residues. We investigated the structure of this giant molecule by X-ray crystallography, circular dichroism (CD) spectrometry, and small-angle X-ray scattering (SAXS). The crystal structure of two repeats showed that each repeat consists of two distinct three-helix bundles, and two such repeats are connected along the long axis, resulting in a rod-like structure that is 120 A in length. CD and SAXS analyses of the samples with longer repeats suggested that each repeat has an identical structure, and that such repeats are connected tandemly to form a rod-like structure in solution, the length of which increased proportionately with the number of repeating units. On the basis of these results, it was proposed that Ebh is a 320 nm rod-like molecule with some plasticity at module junctions.
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(as it appears on PubMed at http://www.pubmed.gov), where 18334223 is the PubMed ID number.
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==About this Structure==
==About this Structure==
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[[Category: Helix-bundle]]
[[Category: Helix-bundle]]
[[Category: Rod-like structure]]
[[Category: Rod-like structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:37:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:34:47 2008''

Revision as of 09:34, 28 July 2008

Image:2dgj.png

Template:STRUCTURE 2dgj

Crystal structure of EbhA (756-1003 domain) from Staphylococcus aureus

Template:ABSTRACT PUBMED 18334223

About this Structure

2DGJ is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus., Tanaka Y, Sakamoto S, Kuroda M, Goda S, Gao YG, Tsumoto K, Hiragi Y, Yao M, Watanabe N, Ohta T, Tanaka I, Structure. 2008 Mar;16(3):488-96. PMID:18334223

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