1re0

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{{STRUCTURE_1re0| PDB=1re0 | SCENE= }}
{{STRUCTURE_1re0| PDB=1re0 | SCENE= }}
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'''Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A'''
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===Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A===
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==Overview==
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ARF GTPases are activated by guanine nucleotide exchange factors (GEFs) of the Sec7 family that promote the exchange of GDP for GTP. Brefeldin A (BFA) is a fungal metabolite that binds to the ARF1*GDP*Sec7 complex and blocks GEF activity at an early stage of the reaction, prior to guanine nucleotide release. The crystal structure of the ARF1*GDP*Sec7*BFA complex shows that BFA binds at the protein-protein interface to inhibit conformational changes in ARF1 required for Sec7 to dislodge the GDP molecule. Based on a comparative analysis of the inhibited complex, nucleotide-free ARF1*Sec7 and ARF1*GDP, we suggest that, in addition to forcing nucleotide release, the ARF1-Sec7 binding energy is used to open a cavity on ARF1 to facilitate the rearrangement of hydrophobic core residues between the GDP and GTP conformations. Thus, the Sec7 domain may act as a dual catalyst, facilitating both nucleotide release and conformational switching on ARF proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 14690595 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14690595}}
==About this Structure==
==About this Structure==
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[[Category: All-helical]]
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[[Category: Alph-beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:22:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:35:41 2008''

Revision as of 09:35, 28 July 2008

Image:1re0.png

Template:STRUCTURE 1re0

Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A

Template:ABSTRACT PUBMED 14690595

About this Structure

1RE0 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism., Mossessova E, Corpina RA, Goldberg J, Mol Cell. 2003 Dec;12(6):1403-11. PMID:14690595

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