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| | {{STRUCTURE_2iss| PDB=2iss | SCENE= }} | | {{STRUCTURE_2iss| PDB=2iss | SCENE= }} |
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| - | '''Structure of the PLP synthase Holoenzyme from Thermotoga maritima'''
| + | ===Structure of the PLP synthase Holoenzyme from Thermotoga maritima=== |
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| - | ==Overview==
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| - | Pyridoxal 5'-phosphate (PLP) is the biologically active form of vitamin B6 and is an important cofactor for several of the enzymes involved in the metabolism of amine-containing natural products such as amino acids and amino sugars. The PLP synthase holoenzyme consists of two subunits: YaaD catalyzes the condensation of ribulose 5-phosphate, glyceraldehyde-3-phosphate, and ammonia, and YaaE catalyzes the production of ammonia from glutamine. Here we describe the structure of the PLP synthase complex (YaaD-YaaE) from Thermotoga maritima at 2.9 A resolution. This complex consists of a core of 12 YaaD monomers with 12 noninteracting YaaE monomers attached to the core. Compared with the previously published structure of PdxS (a YaaD ortholog in Geobacillus stearothermophilus), the N-terminus (1-18), which includes helix alpha0, the beta2-alpha2 loop (46-56), which includes new helix alpha2a, and the C-terminus (270-280) of YaaD are ordered in the complex but disordered in PdxS. A ribulose 5-phosphate is bound to YaaD via an imine with Lys82. Previous studies have demonstrated a similar imine at Lys149 and not at Lys81 (equivalent to Lys150 and Lys82 in T. maritima) for the Bacillus subtilis enzyme suggesting the possibility that two separate sites on YaaD are involved in PLP formation. A phosphate from the crystallization solution is found bound to YaaD and also serves as a marker for a possible second active site. An ammonia channel that connects the active site of YaaE with the ribulose 5-phosphate binding site was identified. This channel is similar to one found in imidazole glycerol phosphate synthase; however, when the beta-barrels of the two complexes are superimposed, the glutaminase domains are rotated by about 180 degrees with respect to each other.
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Zhang, Y.]] | | [[Category: Zhang, Y.]] |
| | [[Category: Alpha/beta three layer sandwich]] | | [[Category: Alpha/beta three layer sandwich]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:49:32 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:39:44 2008'' |
Revision as of 09:39, 28 July 2008
Template:STRUCTURE 2iss
Structure of the PLP synthase Holoenzyme from Thermotoga maritima
Template:ABSTRACT PUBMED 17144654
About this Structure
2ISS is a Protein complex structure of sequences from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
Structural insights into the mechanism of the PLP synthase holoenzyme from Thermotoga maritima., Zein F, Zhang Y, Kang YN, Burns K, Begley TP, Ealick SE, Biochemistry. 2006 Dec 12;45(49):14609-20. PMID:17144654
Page seeded by OCA on Mon Jul 28 12:39:44 2008
