1mpt

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spinqualitylabelsall models 1mpt, resolution 2.4Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF A NEW ALKALINE SERINE PROTEASE (M-PROTEASE) FROM BACILLUS SP. KSM-K16

Overview

An alkaline serine protease, M-protease, from Bacillus sp. KSM-K16 has, been crystallized. Two morphologically different crystal forms were, obtained. Crystal data of form 1: space group P2(1)2(1)2(1), a = 47.3, b =, 62.5, c = 75.6 A, V = 2.23 x 10(5) A(3), Z = 4 and V(m) = 2.09 A(3), Da(-1). Crystal data of form 2: space group P2(1)2(1)2(1), a = 75.82 (2), b = 57.79 (2), c = 54.19 (1) A, V = 2.29 (2) x 10(5) A(3), Z = 4 and V(m), = 2.15 A(3) Da(-1). The crystal structure of M-protease in form 2 has been, solved by molecular replacement using the atomic model of subtilisin, Carlsberg (SBC) which is 60% homologous with M-protease, and refined to, the crystallographic R-factor of 0.189 for 7004 reflections with, F(o)/sigma(F) > 3 between 7 and 2.4 A resolution. The final model of, M-protease contains 1882 protein atoms, two calcium ions and 44 water, molecules. The three-dimensional structure of M-protease is essentially, similar to other subtilisins of known structure. The 269 C(alpha), positions of M-protease have an r.m.s. difference of 1.06 A with the, corresponding positions of SBC. The crystal data of form 2 are close to, those of SBC, though the structure determination of form 2 made it clear, that it is not isomorphous to the crystal structure of SBC. The deletions, of amino acids occur at the residues 36' and 160'-163' compared with SBC, (numerals with primes show the numbering for SBC). The deletion of the, four residues (160'-163') may significantly affect the lack of isomorphism, between M-protease and SBC.

About this Structure

1MPT is a Single protein structure of sequence from Bacillus clausii with CA as ligand. Active as Subtilisin, with EC number 3.4.21.62 Full crystallographic information is available from OCA.

Reference

Structure of a new alkaline serine protease (M-protease) from Bacillus sp. KSM-K16., Yamane T, Kani T, Hatanaka T, Suzuki A, Ashida T, Kobatashi T, Ito S, Yamashita O, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):199-206. PMID:15299321

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