1rh6

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[[Image:1rh6.gif|left|200px]]
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{{STRUCTURE_1rh6| PDB=1rh6 | SCENE= }}
{{STRUCTURE_1rh6| PDB=1rh6 | SCENE= }}
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'''Bacteriophage Lambda Excisionase (Xis)-DNA Complex'''
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===Bacteriophage Lambda Excisionase (Xis)-DNA Complex===
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==Overview==
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The excisionase (Xis) protein from bacteriophage lambda is the best characterized member of a large family of recombination directionality factors that control integrase-mediated DNA rearrangements. It triggers phage excision by cooperatively binding to sites X1 and X2 within the phage, bending DNA significantly and recruiting the phage-encoded integrase (Int) protein to site P2. We have determined the co-crystal structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis forms a unique winged-helix motif that interacts with the major and minor grooves of its binding site using an alpha-helix and an ordered beta-hairpin (wing), respectively. Recognition is achieved through an elaborate water-mediated hydrogen-bonding network at the major groove interface, while the preformed hairpin forms largely non-specific interactions with the minor groove. The structure of the complex provides insights into how Xis recruits Int cooperatively, and suggests a plausible mechanism by which it may distort longer DNA fragments significantly. It reveals a surface on the protein that is likely to mediate Xis-Xis interactions required for its cooperative binding to DNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_15066428}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15066428 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15066428}}
==About this Structure==
==About this Structure==
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[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Site-specific dna recombination]]
[[Category: Site-specific dna recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:29:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:45:53 2008''

Revision as of 09:45, 28 July 2008

Image:1rh6.png

Template:STRUCTURE 1rh6

Bacteriophage Lambda Excisionase (Xis)-DNA Complex

Template:ABSTRACT PUBMED 15066428

About this Structure

1RH6 is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

Reference

Crystal structure of the excisionase-DNA complex from bacteriophage lambda., Sam MD, Cascio D, Johnson RC, Clubb RT, J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:15066428

Page seeded by OCA on Mon Jul 28 12:45:53 2008

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