1p6r

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{{STRUCTURE_1p6r| PDB=1p6r | SCENE= }}
{{STRUCTURE_1p6r| PDB=1p6r | SCENE= }}
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'''Solution structure of the DNA binding domain of the repressor BlaI.'''
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===Solution structure of the DNA binding domain of the repressor BlaI.===
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==Overview==
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beta-Lactamase and penicillin-binding protein PBP2' mediate staphylococcal resistance to beta-lactam antibiotics, which are otherwise highly clinically effective. Two repressors (BlaI and MecI) regulate expression of these inducible proteins. Here, we present the first solution structure of the 82 amino acid residue DNA-binding domain of Bacillus licheniformis BlaI which is very similar in primary sequence to the medically significant Staphyloccocal BlaI and MecI proteins. This structure is composed of a compact core of three alpha-helices and a three-stranded beta-sheet typical of the winged helix protein (WHP) family. The protein/DNA complex was studied by NMR chemical shift comparison between the free and complexed forms of BlaI. Residues involved in DNA interaction were identified and a WHP canonical model of interaction with the operators is proposed. In this model, specific contacts occur between the base-pairs of the TACA motif and conserved amino acid residues of the repressor helix H3. These results help toward understanding the repression and induction mechanism of the genes coding for beta-lactamase and PBP2'.
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(as it appears on PubMed at http://www.pubmed.gov), where 14568532 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14568532}}
==About this Structure==
==About this Structure==
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1P6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6R OCA].
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1P6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6R OCA].
==Reference==
==Reference==
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Winged helix protein]]
[[Category: Winged helix protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:45:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:49:19 2008''

Revision as of 09:49, 28 July 2008

Image:1p6r.png

Template:STRUCTURE 1p6r

Solution structure of the DNA binding domain of the repressor BlaI.

Template:ABSTRACT PUBMED 14568532

About this Structure

1P6R is a Single protein structure of sequence from Bacillus licheniformis. Full experimental information is available from OCA.

Reference

Solution structural study of BlaI: implications for the repression of genes involved in beta-lactam antibiotic resistance., Melckebeke HV, Vreuls C, Gans P, Filee P, Llabres G, Joris B, Simorre JP, J Mol Biol. 2003 Oct 31;333(4):711-20. PMID:14568532

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