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1otj

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{{STRUCTURE_1otj| PDB=1otj | SCENE= }}
{{STRUCTURE_1otj| PDB=1otj | SCENE= }}
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'''Crystal structure of APO (iron-free) TauD'''
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===Crystal structure of APO (iron-free) TauD===
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==Overview==
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The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonate (taurine) to sulfite and aminoacetaldehyde while decomposing alphaKG to succinate and CO(2). This alphaKG dependent dioxygenase is expressed in Escherichia coli under sulfur starvation conditions and allows the cell to utilize taurine, and other similar sulfonates in the environment, as an alternative sulfur source. In this work, we report the structures of the apo and holo forms of TauD to 1.9 A resolution (R(cryst) = 21.2%, R(free) = 24.9%) and 2.5 A resolution (R(cryst) = 22.5%, R(free) = 27.8%), respectively. The models reported herein provide significant new insight into the substrate orientations at the active site and the conformational changes that are induced upon taurine binding. Furthermore, analysis of our crystallographic data coupled with reanalysis of the crystallographic model (resolution = 3.0 A, R(cryst) = 28.1, R(free) = 32.0) presented by Elkins et al. (Biochemistry (2002) 41, 5185-5192) reveals an alternative oligomeric arrangement for the enzyme that is consistent with the conserved primary and secondary structure elements of other alphaKG dependent dioxygenases.
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==About this Structure==
==About this Structure==
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[[Category: Jelly roll motif]]
[[Category: Jelly roll motif]]
[[Category: Taurine]]
[[Category: Taurine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:15:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:02:56 2008''

Revision as of 10:02, 28 July 2008

Image:1otj.png

Template:STRUCTURE 1otj

Crystal structure of APO (iron-free) TauD

Template:ABSTRACT PUBMED 12741810

About this Structure

1OTJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Substrate-induced conformational changes in Escherichia coli taurine/alpha-ketoglutarate dioxygenase and insight into the oligomeric structure., O'Brien JR, Schuller DJ, Yang VS, Dillard BD, Lanzilotta WN, Biochemistry. 2003 May 20;42(19):5547-54. PMID:12741810

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