2ohe

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{{STRUCTURE_2ohe| PDB=2ohe | SCENE= }}
{{STRUCTURE_2ohe| PDB=2ohe | SCENE= }}
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'''Structural and mutational analysis of tRNA-Intron splicing endonuclease from Thermoplasma acidophilum DSM 1728'''
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===Structural and mutational analysis of tRNA-Intron splicing endonuclease from Thermoplasma acidophilum DSM 1728===
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==Overview==
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In archaea, RNA endonucleases that act specifically on RNA with bulge-helix-bulge motifs play the main role in the recognition and excision of introns, while the eukaryal enzymes use a measuring mechanism to determine the positions of the universally positioned splice sites relative to the conserved domain of pre-tRNA. Two crystallographic structures of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728 (EndA(Ta)) have been solved to 2.5-A and 2.7-A resolution by molecular replacement, using the 2.7-A resolution data as the initial model and the single-wavelength anomalous-dispersion phasing method using selenomethionine as anomalous signals, respectively. The models show that EndA(Ta) is a homodimer and that it has overall folding similar to that of other archaeal tRNA endonucleases. From structural and mutational analyses of H236A, Y229F, and K265I in vitro, we have demonstrated that they play critical roles in recognizing the splice site and in cleaving the pre-tRNA substrate.
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==About this Structure==
==About this Structure==
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[[Category: Splicing]]
[[Category: Splicing]]
[[Category: Trna]]
[[Category: Trna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:55:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:03:44 2008''

Revision as of 10:03, 28 July 2008

Image:2ohe.png

Template:STRUCTURE 2ohe

Structural and mutational analysis of tRNA-Intron splicing endonuclease from Thermoplasma acidophilum DSM 1728

Template:ABSTRACT PUBMED 17827289

About this Structure

2OHE is a Single protein structure of sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA.

Reference

Structural and mutational analysis of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728: catalytic mechanism of tRNA intron-splicing endonucleases., Kim YK, Mizutani K, Rhee KH, Nam KH, Lee WH, Lee EH, Kim EE, Park SY, Hwang KY, J Bacteriol. 2007 Nov;189(22):8339-46. Epub 2007 Sep 7. PMID:17827289

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