2rdl

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{{STRUCTURE_2rdl| PDB=2rdl | SCENE= }}
{{STRUCTURE_2rdl| PDB=2rdl | SCENE= }}
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'''Hamster Chymase 2'''
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===Hamster Chymase 2===
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==Overview==
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Divergence of substrate specificity within the context of a common structural framework represents an important mechanism by which new enzyme activity naturally evolves. We present enzymological and x-ray structural data for hamster chymase-2 (HAM2) that provides a detailed explanation for the unusual hydrolytic specificity of this rodent alpha-chymase. In enzymatic characterization, hamster chymase-1 (HAM1) showed typical chymase proteolytic activity. In contrast, HAM2 exhibited atypical substrate specificity, cleaving on the carboxyl side of the P1 substrate residues Ala and Val, characteristic of elastolytic rather than chymotryptic specificity. The 2.5-A resolution crystal structure of HAM2 complexed to the peptidyl inhibitor MeOSuc-Ala-Ala-Pro-Ala-chloromethylketone revealed a narrow and shallow S1 substrate binding pocket that accommodated only a small hydrophobic residue (e.g. Ala or Val). The different substrate specificities of HAM2 and HAM1 are explained by changes in four S1 substrate site residues (positions 189, 190, 216, and 226). Of these, Asn(189), Val(190), and Val(216) form an easily identifiable triplet in all known rodent alpha-chymases that can be used to predict elastolytic specificity for novel chymase-like sequences. Phylogenetic comparison defines guinea pig and rabbit chymases as the closest orthologs to rodent alpha-chymases.
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(as it appears on PubMed at http://www.pubmed.gov), where 17981788 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17981788}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:41:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:04:21 2008''

Revision as of 10:04, 28 July 2008

Image:2rdl.png

Template:STRUCTURE 2rdl

Hamster Chymase 2

Template:ABSTRACT PUBMED 17981788

About this Structure

2RDL is a Single protein structure of sequence from Mesocricetus auratus. Full crystallographic information is available from OCA.

Reference

Structural basis for elastolytic substrate specificity in rodent alpha-chymases., Kervinen J, Abad M, Crysler C, Kolpak M, Mahan AD, Masucci JA, Bayoumy S, Cummings MD, Yao X, Olson M, de Garavilla L, Kuo L, Deckman I, Spurlino J, J Biol Chem. 2008 Jan 4;283(1):427-36. Epub 2007 Oct 31. PMID:17981788

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