2agm

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[[Image:2agm.gif|left|200px]]
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{{Seed}}
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[[Image:2agm.png|left|200px]]
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{{STRUCTURE_2agm| PDB=2agm | SCENE= }}
{{STRUCTURE_2agm| PDB=2agm | SCENE= }}
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'''Solution structure of the R-module from AlgE4'''
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===Solution structure of the R-module from AlgE4===
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==Overview==
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In the bacterium Azotobacter vinelandii, a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1-7) has been identified. These epimerases are responsible for the epimerization of beta-d-mannuronic acid to alpha-l-guluronic acid in alginate polymers. The epimerases consist of two types of structural modules, designated A (one or two copies) and R (one to seven copies). The structure of the catalytically active A-module from the smallest epimerase AlgE4 (consisting of AR) has been solved recently. This paper describes the NMR structure of the R-module from AlgE4 and its titration with a substrate analogue and paramagnetic thulium ions. The R-module folds into a right-handed parallel beta-roll. The overall shape of the R-module is an elongated molecule with a positively charged patch that interacts with the substrate. Titration of the R-module with thulium indicated possible calcium binding sites in the loops formed by the nonarepeat sequences in the N-terminal part of the molecule and the importance of calcium binding for the stability of the R-module. Structure calculations showed that calcium ions can be incorporated in these loops without structural violations and changes. Based on the structure and the electrostatic surface potential of both the A- and R-module from AlgE4, a model for the appearance of the whole protein is proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 16407237 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16407237}}
==About this Structure==
==About this Structure==
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2AGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGM OCA].
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2AGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGM OCA].
==Reference==
==Reference==
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[[Category: Wimmer, R.]]
[[Category: Wimmer, R.]]
[[Category: Parallel beta-roll]]
[[Category: Parallel beta-roll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:01:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:06:28 2008''

Revision as of 10:06, 28 July 2008

Image:2agm.png

Template:STRUCTURE 2agm

Solution structure of the R-module from AlgE4

Template:ABSTRACT PUBMED 16407237

About this Structure

2AGM is a Single protein structure of sequence from Azotobacter vinelandii. Full experimental information is available from OCA.

Reference

NMR structure of the R-module: a parallel beta-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase., Aachmann FL, Svanem BI, Guntert P, Petersen SB, Valla S, Wimmer R, J Biol Chem. 2006 Mar 17;281(11):7350-6. Epub 2006 Jan 3. PMID:16407237

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