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{{STRUCTURE_2gov| PDB=2gov | SCENE= }}
{{STRUCTURE_2gov| PDB=2gov | SCENE= }}
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'''Solution structure of Murine p22HBP'''
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===Solution structure of Murine p22HBP===
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==Overview==
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Murine p22HBP, a 22-kDa monomer originally identified as a cytosolic heme-binding protein ubiquitously expressed in various tissues, has 27% sequence identity to murine SOUL, a heme-binding hexamer specifically expressed in the retina. In contrast to murine SOUL, which binds one heme per subunit via coordination of the Fe(III)-heme to a histidine, murine p22HBP binds one heme molecule per subunit with no specific axial ligand coordination of the Fe(III)-heme. Using intrinsic protein fluorescence quenching, the values for the dissociation constants of p22HBP for hemin and protoporphyrin-IX were determined to be in the low nanomolar range. The three-dimensional structure of murine p22HBP, the first for a protein from the SOUL/HBP family, was determined by NMR methods to consist of a 9-stranded distorted beta-barrel flanked by two long alpha-helices. Although homologous domains have been found in three bacterial proteins, two of which are transcription factors, the fold determined for p22HBP corresponds to a novel alpha plus beta fold in a eukaryotic protein. Chemical shift mapping localized the tetrapyrrole binding site to a hydrophobic cleft formed by residues from helix alphaA and an extended loop. In an attempt to assess the structural basis for tetrapyrrole binding in the SOUL/HBP family, models for the p22HBP-protoporphyrin-IX complex and the SOUL protein were generated by manual docking and automated methods.
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(as it appears on PubMed at http://www.pubmed.gov), where 16905545 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16905545}}
==About this Structure==
==About this Structure==
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2GOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOV OCA].
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2GOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GOV OCA].
==Reference==
==Reference==
The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905545 16905545]
The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905545 16905545]
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1H, 15N and 13C resonance assignments of the heme-binding protein murine p22HBP., Dias JS, Macedo AL, Ferreira GC, Jeanty N, Taketani S, Goodfellow BJ, Peterson FC, Volkman BF, J Biomol NMR. 2005 Aug;32(4):338. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16211492 16211492]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Psi]]
[[Category: Psi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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Revision as of 10:06, 28 July 2008

Image:2gov.png

Template:STRUCTURE 2gov

Solution structure of Murine p22HBP

Template:ABSTRACT PUBMED 16905545

About this Structure

2GOV is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:16905545

1H, 15N and 13C resonance assignments of the heme-binding protein murine p22HBP., Dias JS, Macedo AL, Ferreira GC, Jeanty N, Taketani S, Goodfellow BJ, Peterson FC, Volkman BF, J Biomol NMR. 2005 Aug;32(4):338. PMID:16211492

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