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2e24

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{{STRUCTURE_2e24| PDB=2e24 | SCENE= }}
{{STRUCTURE_2e24| PDB=2e24 | SCENE= }}
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'''crystal structure of a mutant (R612A) of xanthan lyase'''
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===crystal structure of a mutant (R612A) of xanthan lyase===
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==Overview==
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Xanthan is a bacterial heteropolysaccharide composed of pentasaccharide repeating units, i.e., a cellobiose as a backbone and a trisaccharide consisting of two mannoses and one glucuronic acid as a side chain. Nonreducing terminal mannose residues of xanthan side chains are partially pyruvated. Bacillus sp. GL1 xanthan lyase, a member of polysaccharide lyase family 8, acts specifically on pyruvated side chains of xanthan and yields pyruvated mannose through a beta-elimination reaction by using a single Tyr255 residue as base and acid catalysts. Here we show structural factors for substrate recognition by xanthan lyase through X-ray crystallographic and mutational analyses. The enzyme accommodates mannose and pyruvated mannose at the -1 subsite, although both inhibitor and dissociation constants of the two monosaccharides indicated that the affinity of pyruvated mannose for xanthan lyase is much higher than that of mannose. The high affinity of pyruvated mannose is probably due to the formation of additional hydrogen bonds between the carboxyl group of pyruvated mannose and amino acid residues of Tyr315 and Arg612. Site-directed mutagenesis of the two residues demonstrated that Arg612 is a key residue in recognizing pyruvated mannose. Arg612 is located in the protruding loop covering the substrate, suggesting that the loop functions as a lid that is responsible for the proper accommodation of the substrate at the active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 17223699 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17223699}}
==About this Structure==
==About this Structure==
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[[Category: Polysaccharide lyase]]
[[Category: Polysaccharide lyase]]
[[Category: Xanthan]]
[[Category: Xanthan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:46:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:09:01 2008''

Revision as of 10:09, 28 July 2008

Image:2e24.png

Template:STRUCTURE 2e24

crystal structure of a mutant (R612A) of xanthan lyase

Template:ABSTRACT PUBMED 17223699

About this Structure

2E24 is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan., Maruyama Y, Mikami B, Hashimoto W, Murata K, Biochemistry. 2007 Jan 23;46(3):781-91. PMID:17223699

Page seeded by OCA on Mon Jul 28 13:09:01 2008

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