1wns

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{{STRUCTURE_1wns| PDB=1wns | SCENE= }}
{{STRUCTURE_1wns| PDB=1wns | SCENE= }}
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'''Crystal structure of family B DNA polymerase from hyperthermophilic archaeon pyrococcus kodakaraensis KOD1'''
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===Crystal structure of family B DNA polymerase from hyperthermophilic archaeon pyrococcus kodakaraensis KOD1===
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==Overview==
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The crystal structure of family B DNA polymerase from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 (KOD DNA polymerase) was determined. KOD DNA polymerase exhibits the highest known extension rate, processivity and fidelity. We carried out the structural analysis of KOD DNA polymerase in order to clarify the mechanisms of those enzymatic features. Structural comparison of DNA polymerases from hyperthermophilic archaea highlighted the conformational difference in Thumb domains. The Thumb domain of KOD DNA polymerase shows an "opened" conformation. The fingers subdomain possessed many basic residues at the side of the polymerase active site. The residues are considered to be accessible to the incoming dNTP by electrostatic interaction. A beta-hairpin motif (residues 242-249) extends from the Exonuclease (Exo) domain as seen in the editing complex of the RB69 DNA polymerase from bacteriophage RB69. Many arginine residues are located at the forked-point (the junction of the template-binding and editing clefts) of KOD DNA polymerase, suggesting that the basic environment is suitable for partitioning of the primer and template DNA duplex and for stabilizing the partially melted DNA structure in the high-temperature environments. The stabilization of the melted DNA structure at the forked-point may be correlated with the high PCR performance of KOD DNA polymerase, which is due to low error rate, high elongation rate and processivity.
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==About this Structure==
==About this Structure==
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[[Category: Takagi, M.]]
[[Category: Takagi, M.]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 10:10, 28 July 2008

Image:1wns.png

Template:STRUCTURE 1wns

Crystal structure of family B DNA polymerase from hyperthermophilic archaeon pyrococcus kodakaraensis KOD1

Template:ABSTRACT PUBMED 11178906

About this Structure

1WNS is a Single protein structure of sequence from Thermococcus kodakarensis. This structure supersedes the now removed PDB entry 1gcx. Full crystallographic information is available from OCA.

Reference

Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1., Hashimoto H, Nishioka M, Fujiwara S, Takagi M, Imanaka T, Inoue T, Kai Y, J Mol Biol. 2001 Feb 23;306(3):469-77. PMID:11178906

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