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2esf

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{{STRUCTURE_2esf| PDB=2esf | SCENE= }}
{{STRUCTURE_2esf| PDB=2esf | SCENE= }}
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'''Identification of a Novel Non-Catalytic Bicarbonate Binding Site in Eubacterial beta-Carbonic Anhydrase'''
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===Identification of a Novel Non-Catalytic Bicarbonate Binding Site in Eubacterial beta-Carbonic Anhydrase===
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==Overview==
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The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of orthologues from Porphyridium purpureum, Escherichia coli, and Mycobacterium tuberculosis. Here we present the structure of an additional member of that subclass, that from Haemophilus influenzae, as well as detailed kinetic analysis, revealing the correspondence between structural classification and kinetic profile for this subclass. In addition, we identify a unique, noncatalytic binding mode for the substrate bicarbonate that occurs in both the H. influenzae and E. coli enzymes. The kinetic and structural analysis indicates that binding of bicarbonate in this site of the enzyme may modulate its activity by influencing a pH-dependent, cooperative transition between active and inactive forms. We hypothesize that the two structural subclasses of beta-CAs may provide models for the proposed active and inactive forms of the H. influenzae and E. coli enzymes.
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The line below this paragraph, {{ABSTRACT_PUBMED_16584170}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16584170 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16584170}}
==About this Structure==
==About this Structure==
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[[Category: Carbonic anhydrase,metalloenzyme]]
[[Category: Carbonic anhydrase,metalloenzyme]]
[[Category: Zinc coordination]]
[[Category: Zinc coordination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:03:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:14:28 2008''

Revision as of 10:14, 28 July 2008

Image:2esf.png

Template:STRUCTURE 2esf

Identification of a Novel Non-Catalytic Bicarbonate Binding Site in Eubacterial beta-Carbonic Anhydrase

Template:ABSTRACT PUBMED 16584170

About this Structure

2ESF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase., Cronk JD, Rowlett RS, Zhang KY, Tu C, Endrizzi JA, Lee J, Gareiss PC, Preiss JR, Biochemistry. 2006 Apr 11;45(14):4351-61. PMID:16584170

Page seeded by OCA on Mon Jul 28 13:14:28 2008

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