1pbi

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{{STRUCTURE_1pbi| PDB=1pbi | SCENE= }}
{{STRUCTURE_1pbi| PDB=1pbi | SCENE= }}
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'''CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS'''
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===CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS===
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==Overview==
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The trypsin/chymotrypsin inhibitors from winter pea seeds (PsTI) are members of the Bowman-Birk protease inhibitor (BBPI) family. The crystal structure of the isoform PsTI-IVb was determined by molecular replacement at 2.7 A resolution using the X-ray co-ordinates of the soybean inhibitor as a search model. The inhibitor crystallized with a nearly perfect 2-fold symmetric dimer in the asymmetric unit. Although the overall structure is very similar to that seen in other BBPIs, there are notable new structural features. Unlike the previously reported X-ray structures of BBPIs, the structure of PsTI-IVb includes the C-terminal segment of the molecule. The C-terminal tail of each subunit is partly beta-stranded and interacts with the 2-fold symmetry-related subunit, forming a beta-sheet with strands A and B of this subunit. The dimer is mainly stabilized by a large internal hydrogen-bonded network surrounded by two hydrophobic links. Fluorescence anisotropy decay measurements show that residues Tyr59 and Tyr43 are mobile in the picosecond time scale with a large amplitude. The fluorescence study and a molecular model of the simultaneous binding of PsTI-IVb to porcine trypsin and bovine chymotrypsin are compatible only with a monomeric state of the functional molecule in solution.
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(as it appears on PubMed at http://www.pubmed.gov), where 9887273 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9887273}}
==About this Structure==
==About this Structure==
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[[Category: Chymotrypsin inhibitor]]
[[Category: Chymotrypsin inhibitor]]
[[Category: Trypsin inhibitor]]
[[Category: Trypsin inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:54:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:14:38 2008''

Revision as of 10:14, 28 July 2008

Image:1pbi.png

Template:STRUCTURE 1pbi

CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS

Template:ABSTRACT PUBMED 9887273

About this Structure

1PBI is a Single protein structure of sequence from Pisum sativum. Full crystallographic information is available from OCA.

Reference

Dimeric crystal structure of a Bowman-Birk protease inhibitor from pea seeds., Li de la Sierra I, Quillien L, Flecker P, Gueguen J, Brunie S, J Mol Biol. 1999 Jan 22;285(3):1195-207. PMID:9887273

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