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| - | [[Image:1z6b.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1z6b| PDB=1z6b | SCENE= }} | | {{STRUCTURE_1z6b| PDB=1z6b | SCENE= }} |
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| - | '''Crystal structure of Plasmodium falciparum FabZ at 2.1 A'''
| + | ===Crystal structure of Plasmodium falciparum FabZ at 2.1 A=== |
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| - | ==Overview==
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| - | The unique beta-hydroxyacyl-ACP dehydratase in Plasmodium falciparum, PfFabZ, is involved in fatty acid biosynthesis and catalyzes the dehydration of beta-hydroxy fatty acids linked to acyl carrier protein. The structure was solved by single anomalous dispersion (SAD) phasing using a quick-soaking experiment with potassium iodide and refined to a resolution of 2.1 A. The crystal structure represents the first structure of a Plasmodium beta-hydroxyacyl-ACP dehydratase with broad substrate specificity. The asymmetric unit contains a hexamer that appears as a trimer of dimers. Each dimer shows the known "hot dog" fold that has been observed in only a few other protein structures. Each of the two independent active sites in the dimer is formed by equal contributions from both subunits. The active site is mainly hydrophobic and looks like an L-shaped tunnel. The catalytically important amino acids His 133 and Glu 147' (from the other subunit), together with His98', form the only hydrophilic site in this tunnel. The inner end of the active site tunnel is closed by the phenyl ring of Phe 169, which is located in a flexible, partly visible loop. In order to explain the acceptance of substrates longer than ~C-7, the phenyl ring must move away to open the tunnel. The present structure supports an enzymatic mechanism consisting of an elimination reaction catalyzed by His 133 and Glu147'. 3-decynoyl-N-acetylcysteamine, an inhibitor known to interact with the E. coli dehydratase/isomerase, turned out to interact covalently with PfFabZ. A first model of PfFabZ with this potent inhibitor is presented. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15930004}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15930004 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15930004}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Plasmodium falciparum]] | | [[Category: Plasmodium falciparum]] |
| | [[Category: Sad phasing]] | | [[Category: Sad phasing]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:13:35 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:14:53 2008'' |
Revision as of 10:15, 28 July 2008
Template:STRUCTURE 1z6b
Crystal structure of Plasmodium falciparum FabZ at 2.1 A
Template:ABSTRACT PUBMED 15930004
About this Structure
1Z6B is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
The crystal structure of PfFabZ, the unique beta-hydroxyacyl-ACP dehydratase involved in fatty acid biosynthesis of Plasmodium falciparum., Kostrewa D, Winkler FK, Folkers G, Scapozza L, Perozzo R, Protein Sci. 2005 Jun;14(6):1570-80. PMID:15930004
Page seeded by OCA on Mon Jul 28 13:14:53 2008
