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2h25

From Proteopedia

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[[Image:2h25.jpg|left|200px]]
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[[Image:2h25.png|left|200px]]
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{{STRUCTURE_2h25| PDB=2h25 | SCENE= }}
{{STRUCTURE_2h25| PDB=2h25 | SCENE= }}
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'''Solution Structure of Maltose Binding Protein complexed with beta-cyclodextrin'''
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===Solution Structure of Maltose Binding Protein complexed with beta-cyclodextrin===
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==Overview==
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So far high-resolution structure determination by nuclear magnetic resonance (NMR) spectroscopy has been limited to proteins &lt;30 kDa, although global fold determination is possible for substantially larger proteins. Here we present a strategy for assigning backbone and side-chain resonances of large proteins without deuteration, with which one can obtain high-resolution structures from (1)H-(1)H distance restraints. The strategy uses information from through-bond correlation experiments to filter intraresidue and sequential correlations from through-space correlation experiments, and then matches the filtered correlations to obtain sequential assignment. We demonstrate this strategy on three proteins ranging from 24 to 65 kDa for resonance assignment and on maltose binding protein (42 kDa) and hemoglobin (65 kDa) for high-resolution structure determination. The strategy extends the size limit for structure determination by NMR spectroscopy to 42 kDa for monomeric proteins and to 65 kDa for differentially labeled multimeric proteins without the need for deuteration or selective labeling.
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{{ABSTRACT_PUBMED_17060917}}
==About this Structure==
==About this Structure==
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2H25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H25 OCA].
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2H25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H25 OCA].
==Reference==
==Reference==
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[[Category: Zheng, Y.]]
[[Category: Zheng, Y.]]
[[Category: Alpha/beta protein]]
[[Category: Alpha/beta protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:46:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:16:27 2008''

Revision as of 10:16, 28 July 2008

Image:2h25.png

Template:STRUCTURE 2h25

Solution Structure of Maltose Binding Protein complexed with beta-cyclodextrin

Template:ABSTRACT PUBMED 17060917

About this Structure

2H25 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

A new strategy for structure determination of large proteins in solution without deuteration., Xu Y, Zheng Y, Fan JS, Yang D, Nat Methods. 2006 Nov;3(11):931-7. PMID:17060917

Page seeded by OCA on Mon Jul 28 13:16:27 2008

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