2f8t

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[[Image:2f8t.gif|left|200px]]
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{{Seed}}
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[[Image:2f8t.png|left|200px]]
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{{STRUCTURE_2f8t| PDB=2f8t | SCENE= }}
{{STRUCTURE_2f8t| PDB=2f8t | SCENE= }}
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'''Crystal structure of Aa-Ago with externally-bound siRNA'''
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===Crystal structure of Aa-Ago with externally-bound siRNA===
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==Overview==
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Argonaute proteins are key components of the RNA-induced silencing complex (RISC). They provide both architectural and catalytic functionalities associated with small interfering RNA (siRNA) guide strand recognition and subsequent guide strand-mediated cleavage of complementary mRNAs. We report on the 3.0 A crystal structures of 22-mer and 26-mer siRNAs bound to Aquifex aeolicus Argonaute (Aa-Ago), where one 2 nt 3' overhang of the siRNA inserts into a cavity positioned on the outer surface of the PAZ-containing lobe of the bilobal Aa-Ago architecture. The first overhang nucleotide stacks over a tyrosine ring, while the second overhang nucleotide, together with the intervening sugar-phosphate backbone, inserts into a preformed surface cavity. Photochemical crosslinking studies on Aa-Ago with 5-iodoU-labeled single-stranded siRNA and siRNA duplex provide support for this externally bound siRNA-Aa-Ago complex. The structure and biochemical data together provide insights into a protein-RNA recognition event potentially associated with the RISC-loading pathway.
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The line below this paragraph, {{ABSTRACT_PUBMED_17027504}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17027504 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17027504}}
==About this Structure==
==About this Structure==
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[[Category: Risc loading complex]]
[[Category: Risc loading complex]]
[[Category: Sirna]]
[[Category: Sirna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:35:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:24:14 2008''

Revision as of 10:24, 28 July 2008

Image:2f8t.png

Template:STRUCTURE 2f8t

Crystal structure of Aa-Ago with externally-bound siRNA

Template:ABSTRACT PUBMED 17027504

About this Structure

2F8T is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA., Yuan YR, Pei Y, Chen HY, Tuschl T, Patel DJ, Structure. 2006 Oct;14(10):1557-65. PMID:17027504

Page seeded by OCA on Mon Jul 28 13:24:14 2008

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