1s6i

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{{STRUCTURE_1s6i| PDB=1s6i | SCENE= }}
{{STRUCTURE_1s6i| PDB=1s6i | SCENE= }}
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'''Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)'''
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===Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)===
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==Overview==
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Calcium-dependent protein kinases (CDPKs) are an extensive class of multidomain Ca(2+)-regulated enzymes from plants and protozoa. In vivo the so-called calmodulin-like domain (CLD) of CDPK binds intramolecularly to the junction domain (JD), which exhibits both kinase-inhibitory and CLD binding properties. Here we report the high resolution solution structure of the calcium-regulatory region from soybean CDPK-alpha determined in the presence of a peptide encompassing the JD. The structure of both lobes of CLD resembles that of related helix-loop-helix Ca(2+)-binding proteins. NMR chemical shift mapping studies demonstrate that the JD induces significant structural changes in isolated Ca(2+)-CLD, particularly the C-terminal domain, although a stable complex is not formed. A CLD solution structure calculated on the basis of NMR data and long range fluorescence resonance energy transfer distances reveals an activated state with both lobes positioned side by side, similar to calcineurin B rather than calmodulin, highlighting the possible pitfall of assigning function purely from sequence information.
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{{ABSTRACT_PUBMED_15155727}}
==About this Structure==
==About this Structure==
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1S6I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6I OCA].
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1S6I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6I OCA].
==Reference==
==Reference==
Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha., Weljie AM, Vogel HJ, J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155727 15155727]
Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha., Weljie AM, Vogel HJ, J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155727 15155727]
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Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15568805 15568805]
[[Category: Glycine max]]
[[Category: Glycine max]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Helix-loop-helix]]
[[Category: Helix-loop-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:21:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:34:57 2008''

Revision as of 10:34, 28 July 2008

Image:1s6i.png

Template:STRUCTURE 1s6i

Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)

Template:ABSTRACT PUBMED 15155727

About this Structure

1S6I is a Single protein structure of sequence from Glycine max. Full experimental information is available from OCA.

Reference

Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha., Weljie AM, Vogel HJ, J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:15155727

Solution structure and backbone dynamics of the N-terminal region of the calcium regulatory domain from soybean calcium-dependent protein kinase alpha., Weljie AM, Gagne SM, Vogel HJ, Biochemistry. 2004 Dec 7;43(48):15131-40. PMID:15568805

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