1s05

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{{STRUCTURE_1s05| PDB=1s05 | SCENE= }}
{{STRUCTURE_1s05| PDB=1s05 | SCENE= }}
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'''NMR-validated structural model for oxidized R.palustris cytochrome c556'''
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===NMR-validated structural model for oxidized R.palustris cytochrome c556===
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==Overview==
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The structure of oxidized Rhodopseudomonas palustris cytochrome c(556) has been modeled after that of high-spin cytochrome c' from the same bacterium, the latter being the protein with the greatest sequence identity (35%) among all sequenced proteins in the genomes. The two proteins differ in the number of ligands to iron and in spin state, the former being six-coordinate low-spin and the latter five-coordinate high-spin. In order to validate this modeled structure, several structural restraints were obtained by performing a restricted set of NMR experiments, without performing a complete assignment of the protein signals. The aim was to exploit the special restraints arising from the paramagnetism of the metal ion. A total of 43 residual-dipolar-coupling and 74 pseudocontact-shift restraints, which together sampled all regions of the protein, were used in conjunction with over 40 routinely obtained NOE distance restraints. A calculation procedure was undertaken combining the program MODELLER and the solution structure determination program PARAMAGNETIC DYANA, which includes paramagnetism-based restraints. The directions and magnitude of the magnetic susceptibility anisotropy tensor were also calculated. The approach readily provides useful results, especially for paramagnetic metalloproteins of moderate to large dimensions.
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{{ABSTRACT_PUBMED_14735333}}
==About this Structure==
==About this Structure==
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1S05 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S05 OCA].
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1S05 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S05 OCA].
==Reference==
==Reference==
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[[Category: Winkler, J R.]]
[[Category: Winkler, J R.]]
[[Category: This is a model obtained by nmr-restrained modeling and minimization.]]
[[Category: This is a model obtained by nmr-restrained modeling and minimization.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:07:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:35:00 2008''

Revision as of 10:35, 28 July 2008

Image:1s05.png

Template:STRUCTURE 1s05

NMR-validated structural model for oxidized R.palustris cytochrome c556

Template:ABSTRACT PUBMED 14735333

About this Structure

1S05 is a Single protein structure of sequence from Rhodopseudomonas palustris. Full experimental information is available from OCA.

Reference

NMR-validated structural model for oxidized Rhodopseudomonas palustris cytochrome c(556)., Bertini I, Faraone-Mennella J, Gray HB, Luchinat C, Parigi G, Winkler JR, J Biol Inorg Chem. 2004 Mar;9(2):224-30. Epub 2004 Jan 20. PMID:14735333

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