2oj8

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[[Image:2oj8.gif|left|200px]]
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{{Seed}}
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[[Image:2oj8.png|left|200px]]
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{{STRUCTURE_2oj8| PDB=2oj8 | SCENE= }}
{{STRUCTURE_2oj8| PDB=2oj8 | SCENE= }}
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'''NMR structure of the UGUU tetraloop of Duck Epsilon apical stem loop of the Hepatitis B virus'''
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===NMR structure of the UGUU tetraloop of Duck Epsilon apical stem loop of the Hepatitis B virus===
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==Overview==
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Hepatitis B virus (HBV) replication is initiated by binding of its reverse transcriptase (P) to the apical stem-loop (AL) and primer loop (PL) of epsilon, a highly conserved RNA element at the 5'-end of the RNA pregenome. Mutation studies on duck/heron and human in vitro systems have shown similarities but also differences between their P-epsilon interaction. Here, NMR and UV thermodynamic data on AL (and PL) from these three species are presented. The stabilities of the duck and heron ALs were found to be similar, and much lower than that of human. NMR data show that this low stability stems from an 11-nt internal bulge destabilizing the stem of heron AL. In duck, although structured at low temperature, this region also forms a weak point as its imino resonances broaden to disappearance between 30 and 35 degrees C well below the overall AL melting temperature. Surprisingly, the duck- and heron ALs were both found to be capped by a stable well-structured UGUU tetraloop. All avian ALs are expected to adhere to this because of their conserved sequence. Duck PL is stable and structured and, in view of sequence similarities, the same is expected for heron - and human PL.
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The line below this paragraph, {{ABSTRACT_PUBMED_17430968}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17430968 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17430968}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJ8 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJ8 OCA].
==Reference==
==Reference==
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[[Category: Tetraloop]]
[[Category: Tetraloop]]
[[Category: Uguu]]
[[Category: Uguu]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:01:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:35:23 2008''

Revision as of 10:35, 28 July 2008

Image:2oj8.png

Template:STRUCTURE 2oj8

NMR structure of the UGUU tetraloop of Duck Epsilon apical stem loop of the Hepatitis B virus

Template:ABSTRACT PUBMED 17430968

About this Structure

Full experimental information is available from OCA.

Reference

Thermodynamics and NMR studies on Duck, Heron and Human HBV encapsidation signals., Girard FC, Ottink OM, Ampt KA, Tessari M, Wijmenga SS, Nucleic Acids Res. 2007;35(8):2800-11. Epub 2007 Apr 11. PMID:17430968

Page seeded by OCA on Mon Jul 28 13:35:23 2008

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