1mvq

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Revision as of 19:37, 20 November 2007

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spinqualitylabelsall models 1mvq, resolution 1.77Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Cratylia mollis lectin (isoform 1) in complex with methyl-alpha-D-mannose

Overview

Carbohydrate-protein interactions play a key role in many biological, processes. Cramoll is a lectin purified from Cratylia mollis seeds that is, taxonomically related to concanavalin A (Con A). Although Cramoll and Con, A have the same monosaccharide specificity, they have different, glycoprotein binding profiles. We report the primary structure of Cramoll, determined by Edman degradation and mass spectrometry and its 1.77 A, crystallographic structure and compare it with the three-dimensional, structure of Con A in an attempt to understand how differential binding, can be achieved by similar or nearly identical structures. We report here, that Cramoll consists of 236 residues, with 82% identity with Con A, and, that its topological architecture is essentially identical to Con A, because the Calpha positional differences are below 3.5 A. Cramoll and Con, A have identical binding sites for MealphaMan, Mn2+, and Ca2+. However, we, observed six substitutions in a groove adjacent to the extended binding, site and two in the extended binding site that may explain the differences, in binding of oligosaccharides and glycoproteins between Cramoll and Con, A.

About this Structure

1MVQ is a Single protein structure of sequence from Cratylia mollis with MMA, CA and MN as ligands. Full crystallographic information is available from OCA.

Reference

Amino acid sequence and tertiary structure of Cratylia mollis seed lectin., De Souza GA, Oliveira PS, Trapani S, Santos AC, Rosa JC, Laure HJ, Faca VM, Correia MT, Tavares GA, Oliva G, Coelho LC, Greene LJ, Glycobiology. 2003 Dec;13(12):961-72. Epub 2003 Sep 9. PMID:12966038

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