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| - | [[Image:1rdr.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1rdr| PDB=1rdr | SCENE= }} | | {{STRUCTURE_1rdr| PDB=1rdr | SCENE= }} |
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| - | '''POLIOVIRUS 3D POLYMERASE'''
| + | ===POLIOVIRUS 3D POLYMERASE=== |
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| - | ==Overview==
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| - | BACKGROUND: The central player in the replication of RNA viruses is the viral RNA-dependent RNA polymerase. The 53 kDa poliovirus polymerase, together with other viral and possibly host proteins, carries out viral RNA replication in the host cell cytoplasm. RNA-dependent RNA polymerases comprise a distinct category of polymerases that have limited sequence similarity to reverse transcriptases (RNA-dependent DNA polymerases) and perhaps also to DNA-dependent polymerases. Previously reported structures of RNA-dependent DNA polymerases, DNA-dependent DNA polymerases and a DNA-dependent RNA polymerase show that structural and evolutionary relationships exist between the different polymerase categories. RESULTS: We have determined the structure of the RNA-dependent RNA polymerase of poliovirus at 2.6 A resolution by X-ray crystallography. It has the same overall shape as other polymerases, commonly described by analogy to a right hand. The structures of the 'fingers' and 'thumb' subdomains of poliovirus polymerase differ from those of other polymerases, but the palm subdomain contains a core structure very similar to that of other polymerases. This conserved core structure is composed of four of the amino acid sequence motifs described for RNA-dependent polymerases. Structure-based alignments of these motifs has enabled us to modify and extend previous sequence and structural alignments so as to relate sequence conservation to function. Extensive regions of polymerase-polymerase interactions observed in the crystals suggest an unusual higher order structure that we believe is important for polymerase function. CONCLUSIONS: As a first example of a structure of an RNA-dependent RNA polymerase, the poliovirus polymerase structure provides for a better understanding of polymerase structure, function and evolution. In addition, it has yielded insights into an unusual higher order structure that may be critical for poliovirus polymerase function.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9309225}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9309225 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9309225}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Polymerase]] | | [[Category: Polymerase]] |
| | [[Category: Rna-dependent]] | | [[Category: Rna-dependent]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:22:19 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:53:21 2008'' |
Revision as of 10:53, 28 July 2008
Template:STRUCTURE 1rdr
POLIOVIRUS 3D POLYMERASE
Template:ABSTRACT PUBMED 9309225
About this Structure
1RDR is a Single protein structure of sequence from Human poliovirus 1. Full crystallographic information is available from OCA.
Reference
Structure of the RNA-dependent RNA polymerase of poliovirus., Hansen JL, Long AM, Schultz SC, Structure. 1997 Aug 15;5(8):1109-22. PMID:9309225
Page seeded by OCA on Mon Jul 28 13:53:21 2008
