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1ooa

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[[Image:1ooa.gif|left|200px]]
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{{STRUCTURE_1ooa| PDB=1ooa | SCENE= }}
{{STRUCTURE_1ooa| PDB=1ooa | SCENE= }}
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'''CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER'''
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===CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER===
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==Overview==
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We have recently identified an RNA aptamer for the transcription factor NF-kappaB p50 homodimer [(p50)2], which exhibits little sequence resemblance to the consensus DNA target for (p50)2, but binds (p50)2 with an affinity similar to that of the optimal DNA target. We describe here the 2.45-A resolution x-ray crystal structure of the p50 RHR/RNA aptamer complex. The structure reveals that two RNA molecules bind independent of each other to the p50 N-terminal Ig-like domains. The RNA secondary structure is comprised of a stem and a stem-loop separated by an internal loop folded into a kinked helix because of the cross-strand stacking of three internal loop guanines. These guanines, placed at the edge of the 3' helix, together with the major groove of the irregular 3' helix, form the binding surface for p50. Each p50 monomer uses the same surface to recognize the distorted RNA major groove as observed in the kappaB DNA/p50 RHR complex, resulting in strikingly similar interfaces. The structure reveals how the aptamer specifically selects p50 and discriminates against p65. We also discuss the physiological implications of RNA binding by (p50)2.
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(as it appears on PubMed at http://www.pubmed.gov), where 12886018 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12886018}}
==About this Structure==
==About this Structure==
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[[Category: Protein-rna complex]]
[[Category: Protein-rna complex]]
[[Category: Transcription factor nf-kb]]
[[Category: Transcription factor nf-kb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:05:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:00:17 2008''

Revision as of 11:00, 28 July 2008

Image:1ooa.png

Template:STRUCTURE 1ooa

CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER

Template:ABSTRACT PUBMED 12886018

About this Structure

1OOA is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of NF-kappaB (p50)2 complexed to a high-affinity RNA aptamer., Huang DB, Vu D, Cassiday LA, Zimmerman JM, Maher LJ 3rd, Ghosh G, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9268-73. Epub 2003 Jul 28. PMID:12886018

Page seeded by OCA on Mon Jul 28 14:00:17 2008

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