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2g16

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{{STRUCTURE_2g16| PDB=2g16 | SCENE= }}
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'''Structure of S65A Y66S GFP variant after backbone fragmentation'''
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===Structure of S65A Y66S GFP variant after backbone fragmentation===
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==Overview==
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The green fluorescent protein (GFP) creates a fluorophore out of three sequential amino acids by promoting spontaneous posttranslational modifications. Here, we use high-resolution crystallography to characterize GFP variants that not only undergo peptide backbone cyclization but additional denaturation-induced peptide backbone fragmentation, native peptide hydrolysis, and decarboxylation reactions. Our analyses indicate that architectural features that favor GFP peptide cyclization also drive peptide hydrolysis. These results are relevant for the maturation pathways of GFP homologues, such as the kindling fluorescent protein and the Kaede protein, which use backbone cleavage to red-shift the spectral properties of their chromophores. We further propose a photochemical mechanism for the decarboxylation reaction, supporting a role for the GFP protein environment in facilitating radical formation and one-electron chemistry, which may be important in activating oxygen for the oxidation step of chromophore biosynthesis. Together, our results characterize GFP posttranslational modification chemistry with implications for the energetic landscape of backbone cyclization and subsequent reactions, and for the rational design of predetermined spontaneous backbone cyclization and cleavage reactions.
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(as it appears on PubMed at http://www.pubmed.gov), where 16594705 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16594705}}
==About this Structure==
==About this Structure==
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[[Category: Denaturation]]
[[Category: Denaturation]]
[[Category: Fragmentaion]]
[[Category: Fragmentaion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:34:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:02:38 2008''

Revision as of 11:02, 28 July 2008

Image:2g16.png

Template:STRUCTURE 2g16

Structure of S65A Y66S GFP variant after backbone fragmentation

Template:ABSTRACT PUBMED 16594705

About this Structure

2G16 is a Protein complex structure of sequences from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Understanding GFP posttranslational chemistry: structures of designed variants that achieve backbone fragmentation, hydrolysis, and decarboxylation., Barondeau DP, Kassmann CJ, Tainer JA, Getzoff ED, J Am Chem Soc. 2006 Apr 12;128(14):4685-93. PMID:16594705

Page seeded by OCA on Mon Jul 28 14:02:38 2008

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