2ie8

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{{STRUCTURE_2ie8| PDB=2ie8 | SCENE= }}
{{STRUCTURE_2ie8| PDB=2ie8 | SCENE= }}
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'''Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation'''
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===Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation===
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==Overview==
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Phosphoglycerate kinase (PGK) is a key glycolytic enzyme that catalyzes the reversible transfer of a phosphate from 1,3-bisphosphoglycerate to ADP to form 3-phosphoglycerate and ATP in the presence of magnesium. During catalysis, a conformational change occurs that brings the N- and C-domains of PGK closer together. Here we present the 1.8A crystal structure of unliganded PGK from Thermus caldophilus (Tca). Comparison of the structure of TcaPGK (open conformation) with that of Thermotoga maritima (Tma) PGK (closed conformation) revealed that the conformational change reflects a change in the interaction between the domains. We identified Arg148 as a key residue involved in open-to-closed transition. The open conformation of TcaPGK is stabilized by an interdomain salt bridge between Arg148 and Glu375. The binding of 3-PG (or maybe 1,3-BPG) disrupts this salt bridge and, in ternary complex, the formation of new salt bridge between Arg60 and Asp197 stabilizes the closed conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 17045964 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17045964}}
==About this Structure==
==About this Structure==
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[[Category: Phosphoglycerate kinase]]
[[Category: Phosphoglycerate kinase]]
[[Category: Thermus caldophilus]]
[[Category: Thermus caldophilus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:23:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:02:47 2008''

Revision as of 11:02, 28 July 2008

Image:2ie8.png

Template:STRUCTURE 2ie8

Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation

Template:ABSTRACT PUBMED 17045964

About this Structure

2IE8 is a Single protein structure of sequence from Thermus caldophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation., Lee JH, Im YJ, Bae J, Kim D, Kim MK, Kang GB, Lee DS, Eom SH, Biochem Biophys Res Commun. 2006 Dec 1;350(4):1044-9. Epub 2006 Oct 6. PMID:17045964

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