1umh

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{{STRUCTURE_1umh| PDB=1umh | SCENE= }}
{{STRUCTURE_1umh| PDB=1umh | SCENE= }}
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'''Structural basis of sugar-recognizing ubiquitin ligase'''
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===Structural basis of sugar-recognizing ubiquitin ligase===
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==Overview==
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SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.
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(as it appears on PubMed at http://www.pubmed.gov), where 14990996 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14990996}}
==About this Structure==
==About this Structure==
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[[Category: Ubiquitin]]
[[Category: Ubiquitin]]
[[Category: Ubiquitin ligase]]
[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:05:43 2008''

Revision as of 11:05, 28 July 2008

Image:1umh.png

Template:STRUCTURE 1umh

Structural basis of sugar-recognizing ubiquitin ligase

Template:ABSTRACT PUBMED 14990996

About this Structure

1UMH is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:14990996

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