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| | {{STRUCTURE_2a5c| PDB=2a5c | SCENE= }} | | {{STRUCTURE_2a5c| PDB=2a5c | SCENE= }} |
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| - | '''Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine'''
| + | ===Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine=== |
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| - | ==Overview==
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| - | Oxidative damage of DNA results in the formation of many products, including 8-oxodeoxyguanosine, which has been used as a marker to quantify DNA damage. Earlier studies have demonstrated that avidin, a protein prevalent in egg-white and which has high affinity for the vitamin biotin, binds to 8-oxodeoxyguanosine and related bases. In this study, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeoxyadenosine. In each case, the base is observed to bind within the biotin-binding site of avidin. However, the mode of association between the bases and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein in this region does not accompany binding. Fluorescence studies indicate that in solution the individual bases, and a range of oligonucleotides, bind to avidin with micromolar affinity. Only one of the modes of binding observed is consistent with recognition of oxidised purines when incorporated within a DNA oligomer, and from this structure a model is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanosine. These studies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the low levels of binding observed are inconsistent with the recognition of oxidised purines forming a major physiological role for avidin.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16413579}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16413579 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16413579}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Damaged dna]] | | [[Category: Damaged dna]] |
| | [[Category: X-ray crystallography]] | | [[Category: X-ray crystallography]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:30 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:06:24 2008'' |
Revision as of 11:06, 28 July 2008
Template:STRUCTURE 2a5c
Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine
Template:ABSTRACT PUBMED 16413579
About this Structure
2A5C is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Recognition of oxidatively modified bases within the biotin-binding site of avidin., Conners R, Hooley E, Clarke AR, Thomas S, Brady RL, J Mol Biol. 2006 Mar 17;357(1):263-74. Epub 2006 Jan 6. PMID:16413579
Page seeded by OCA on Mon Jul 28 14:06:24 2008
