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2d31

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{{STRUCTURE_2d31| PDB=2d31 | SCENE= }}
{{STRUCTURE_2d31| PDB=2d31 | SCENE= }}
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'''Crystal structure of disulfide-linked HLA-G dimer'''
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===Crystal structure of disulfide-linked HLA-G dimer===
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==Overview==
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HLA-G is a nonclassical major histocompatibility complex class I (MHCI) molecule, which is expressed in trophoblasts and confers immunological tolerance in the maternal-fetal interface by binding to leukocyte Ig-like receptors (LILRs, also called as LIR/ILT/CD85) and CD8. HLA-G is expressed in disulfide-linked dimer form both in solution and at the cell surface. Interestingly, MHCI dimer formations have been involved in pathogenesis and T cell activation. The structure and receptor binding characteristics of MHCI dimers have never been evaluated. Here we performed binding studies showing that the HLA-G dimer exhibited higher overall affinity to LILRB1/2 than the monomer by significant avidity effects. Furthermore, the cell reporter assay demonstrated that the dimer formation remarkably enhanced the LILRB1-mediated signaling at the cellular level. We further determined the crystal structure of the wild-type dimer of HLA-G with the intermolecular Cys(42)-Cys(42) disulfide bond. This dimer structure showed the oblique configuration to expose two LILR/CD8-binding sites upward from the membrane easily accessible for receptors, providing plausible 1:2 (HLA-G dimer:receptors) complex models. These results indicated that the HLA-G dimer conferred increased avidity in a proper structural orientation to induce efficient LILR signaling, resulting in the dominant immunosuppressive effects. Moreover, structural and functional implications for other MHCI dimers observed in activated T cells and the pathogenic allele, HLA-B27, are discussed.
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{{ABSTRACT_PUBMED_16455647}}
==About this Structure==
==About this Structure==
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[[Category: Tsumoto, K.]]
[[Category: Tsumoto, K.]]
[[Category: Mhc class i]]
[[Category: Mhc class i]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:36:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:14:35 2008''

Revision as of 11:14, 28 July 2008

Image:2d31.png

Template:STRUCTURE 2d31

Crystal structure of disulfide-linked HLA-G dimer

Template:ABSTRACT PUBMED 16455647

About this Structure

2D31 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer., Shiroishi M, Kuroki K, Ose T, Rasubala L, Shiratori I, Arase H, Tsumoto K, Kumagai I, Kohda D, Maenaka K, J Biol Chem. 2006 Apr 14;281(15):10439-47. Epub 2006 Feb 2. PMID:16455647

Page seeded by OCA on Mon Jul 28 14:14:35 2008

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