1zbz

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[[Image:1zbz.gif|left|200px]]
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{{STRUCTURE_1zbz| PDB=1zbz | SCENE= }}
{{STRUCTURE_1zbz| PDB=1zbz | SCENE= }}
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'''High-Resolution Crystal Structure of Compound I intermediate of Cytochrome c Peroxidase (CcP)'''
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===High-Resolution Crystal Structure of Compound I intermediate of Cytochrome c Peroxidase (CcP)===
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==Overview==
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Cytochrome c peroxidase (CCP) is a 32.5 kDa mitochondrial intermembrane space heme peroxidase from Saccharomyces cerevisiae that reduces H(2)O(2) to 2H(2)O by oxidizing two molecules of cytochrome c (cyt c). Here we compare the 1.2 A native structure (CCP) with the 1.3 A structure of its stable oxidized reaction intermediate, Compound I (CCP1). In addition, crystals were analyzed by UV-vis absorption and electron paramagnetic resonance spectroscopies before and after data collection to determine the state of the Fe(IV) center and the cationic Trp191 radical formed in Compound I. The results show that X-ray exposure does not lead to reduction of Fe(IV) and only partial reduction of the Trp radical. A comparison of the two structures reveals subtle but important conformational changes that aid in the stabilization of the Trp191 cationic radical in Compound I. The higher-resolution data also enable a more accurate determination of changes in heme parameters. Most importantly, when one goes from resting state Fe(III) to Compound I, the His-Fe bond distance increases, the iron moves into the porphyrin plane leading to shorter pyrrole N-Fe bonds, and the Fe(IV)-O bond distance is 1.87 A, suggesting a single Fe(IV)-O bond and not the generally accepted double bond.
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(as it appears on PubMed at http://www.pubmed.gov), where 12741816 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12741816}}
==About this Structure==
==About this Structure==
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[[Category: High-spin heme]]
[[Category: High-spin heme]]
[[Category: Trp191 cation radical]]
[[Category: Trp191 cation radical]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:26:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:16:41 2008''

Revision as of 11:16, 28 July 2008

Image:1zbz.png

Template:STRUCTURE 1zbz

High-Resolution Crystal Structure of Compound I intermediate of Cytochrome c Peroxidase (CcP)

Template:ABSTRACT PUBMED 12741816

About this Structure

1ZBZ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structures and spectroscopy of native and compound I cytochrome c peroxidase., Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee DE, Goodin DB, Poulos TL, Biochemistry. 2003 May 20;42(19):5600-8. PMID:12741816

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