1myu
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(New page: 200px<br /><applet load="1myu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1myu" /> '''Lipid induced conformation of the tachykinin...)
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Revision as of 19:41, 20 November 2007
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Lipid induced conformation of the tachykinin peptide Kassinin
Overview
Both the aqueous and lipid-induced structure of Kassinin, a dodecapeptide, of amphibian origin, has been studied by two-dimensional proton nuclear, magnetic resonance (2D 1H-NMR) spectroscopy and distance geometry, calculations. Unambiguous NMR assignments of protons have been made with, the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and, nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The, distance constraints obtained from the NMR data have been utilized in a, distance geometry algorithm to generate a family of structures, which have, been refined using restrained energy minimization and dynamics. These data, show that, while in water Kassinin prefers to be in an extended chain, conformation, in the presence of perdeuterated dodecylphosphocholine (DPC), micelles, a membrane model system, helical conformation is induced in the, central core and C-terminal region (K4-M12) of the peptide. N-terminus, though less defined also displays some degree of order and a possible turn, structure. The conformation adopted by Kassinin in the presence of DPC, micelles is consistent with the structural motif typical of neurokinin-1, selective agonists and with that reported for Eledoisin in hydrophobic, environment.
About this Structure
1MYU is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Lipid induced conformation of the tachykinin peptide Kassinin., Grace RC, Lynn AM, Cowsik SM, J Biomol Struct Dyn. 2001 Feb;18(4):611-21, 623-5. PMID:11245256
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