1t50

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{{STRUCTURE_1t50| PDB=1t50 | SCENE= }}
{{STRUCTURE_1t50| PDB=1t50 | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN'''
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===NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN===
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==Overview==
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Water-borne protein pheromones are essential for coordination of reproductive activities in many marine organisms. In this paper, we describe the first structure of a pheromone protein from a marine organism, that of attractin (58 residues) from Aplysia californica. The NMR solution structure was determined from TOCSY, NOESY, and DQF-COSY measurements of recombinant attractin expressed in insect cells. The sequential resonance assignments were done with standard manual procedures. Approximately 90% of the 949 unambiguous NOESY cross-peaks were assigned automatically with simultaneous three-dimensional structure calculation using our NOAH/DIAMOD/FANTOM program suite. The final bundle of energy-refined structures is well-defined, with an average rmsd value to the mean structure of 0.72 +/- 0.12 A for backbone and 1.32 +/- 0.11 A for heavy atoms for amino acids 3-47. Attractin contains two antiparallel helices, made up of residues Ile9-Gln16 and I30-S36. The NMR distance constraints are consistent with the three disulfide bonds determined by mass spectroscopy (C4-C41, C13-C33, and C20-C26), where the first two could be directly determined from NOESY cross-peaks between CH beta protons of the corresponding cysteines. The second helix contains the (L/I)(29)IEECKTS(36) sequence conserved in attractins from five species of Aplysia that could interact with the receptor. The sequence and structure of this region are similar to those of the recognition helix of the Er-11 pheromone of the unicellular ciliate Euplotes raikovi, suggesting a possible common pathway for intercellular communication of these two distinct pheromone families.
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(as it appears on PubMed at http://www.pubmed.gov), where 12924946 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12924946}}
==About this Structure==
==About this Structure==
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1T50 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nj7 1nj7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T50 OCA].
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1T50 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nj7 1nj7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T50 OCA].
==Reference==
==Reference==
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[[Category: Mollusk]]
[[Category: Mollusk]]
[[Category: Pheromone]]
[[Category: Pheromone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:32:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:18:42 2008''

Revision as of 11:18, 28 July 2008

Image:1t50.png

Template:STRUCTURE 1t50

NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN

Template:ABSTRACT PUBMED 12924946

About this Structure

1T50 is a Single protein structure of sequence from Aplysia californica. This structure supersedes the now removed PDB entry 1nj7. Full experimental information is available from OCA.

Reference

NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica., Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W, Biochemistry. 2003 Aug 26;42(33):9970-9. PMID:12924946

Page seeded by OCA on Mon Jul 28 14:18:42 2008

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