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1xlt

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{{STRUCTURE_1xlt| PDB=1xlt | SCENE= }}
{{STRUCTURE_1xlt| PDB=1xlt | SCENE= }}
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'''Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex'''
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===Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex===
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==Overview==
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Transhydrogenase (TH) couples direct and stereospecific hydride transfer between NAD(H) and NADP(H), bound within soluble domains I and III, respectively, to proton translocation across membrane bound domain II. The cocrystal structure of Rhodospirillum rubrum TH domains I and III has been determined in the presence of limiting NADH, under conditions in which the subunits reach equilibrium during crystallization. The crystals contain three heterotrimeric complexes, dI(2)dIII, in the asymmetric unit. Multiple conformations of loops and side-chains, and NAD(H) cofactors, are observed in domain I pertaining to substrate/product exchange, and highlighting electrostatic interactions during the hydride transfer. Two interacting NAD(H)-NADPH pairs are observed where alternate conformations of the NAD(H) phosphodiester and conserved arginine side-chains are correlated. In addition, the stereochemistry of one NAD(H)-NADPH pair approaches that expected for nicotinamide hydride transfer reactions. The cocrystal structure exhibits non-crystallographic symmetry that implies another orientation for domain III, which could occur in dimeric TH. Superposition of the "closed" form of domain III (PDB 1PNO, chain A) onto the dI(2)dIII complex reveals a severe steric conflict of highly conserved loops in domains I and III. This overlap, and the overlap with a 2-fold related domain III, suggests that motions of loop D within domain III and of the entire domain are correlated during turnover. The results support the concept that proton pumping in TH is driven by the difference in binding affinity for oxidized and reduced nicotinamide cofactors, and in the absence of a difference in redox potential, must occur through conformational effects.
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==About this Structure==
==About this Structure==
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[[Category: Nadph]]
[[Category: Nadph]]
[[Category: Transhydrogenase]]
[[Category: Transhydrogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:18:47 2008''

Revision as of 11:18, 28 July 2008

Image:1xlt.png

Template:STRUCTURE 1xlt

Crystal structure of Transhydrogenase [(domain I)2:domain III] heterotrimer complex

Template:ABSTRACT PUBMED 15670609

About this Structure

1XLT is a Protein complex structure of sequences from Rhodospirillum rubrum. Full crystallographic information is available from OCA.

Reference

Conformational diversity in NAD(H) and interacting transhydrogenase nicotinamide nucleotide binding domains., Sundaresan V, Chartron J, Yamaguchi M, Stout CD, J Mol Biol. 2005 Feb 18;346(2):617-29. Epub 2004 Dec 30. PMID:15670609

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