1mzm

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Revision as of 19:42, 20 November 2007

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spinqualitylabelsall models 1mzm, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

MAIZE NONSPECIFIC LIPID TRANSFER PROTEIN COMPLEXED WITH PALMITATE

Overview

BACKGROUND: The movement of lipids between membranes is aided by, lipid-transfer proteins (LTPs). Some LTPs exhibit broad specificity, transferring many classes of lipids, and are termed non-specific LTPs, (ns-LTPs). Despite their apparently similar mode of action, no sequence, homology exists between mammalian and plant ns-LTPs and no, three-dimensional structure has been reported for any plant ns-LTP., RESULTS: We have determined the crystal structure of ns-LTP from maize, seedlings by multiple isomorphous replacement and refined the structure to, 1.9 A resolution. The protein comprises a single compact domain with four, alpha-helices and a long C-terminal region. The eight conserved cysteines, form four disulfide bridges (assigned as Cys4-Cys52, Cys14-Cys29, Cys30-Cys75, and Cys50-Cys89) resolving the ambiguity that remained from, the chemical determination of pairings in the homologous protein from, castor bean. Two of the bonds, Cys4-Cys52 and Cys50-Cys89, differ from, what would have been predicted from sequence alignment with soybean, hydrophobic protein. The complex between maize ns-LTP and hexadecanoate, (palmitate) has also been crystallized and its structure refined to 1.8 A, resolution. CONCLUSIONS: The fold of maize ns-LTP places it in a new, category of all-alpha-type structure, first described for soybean, hydrophobic protein. In the absence of a bound ligand, the protein has a, tunnel-like hydrophobic cavity, which is large enough to accommodate a, long fatty acyl chain. In the structure of the complex with palmitate, most of the acyl chain is buried inside this hydrophobic cavity.

About this Structure

1MZM is a Single protein structure of sequence from Zea mays with PLM and FMT as ligands. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings., Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW, Structure. 1995 Feb 15;3(2):189-99. PMID:7735835

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