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2bcn

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{{STRUCTURE_2bcn| PDB=2bcn | SCENE= }}
{{STRUCTURE_2bcn| PDB=2bcn | SCENE= }}
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'''Solvent isotope effects on interfacial protein electron transfer between cytochrome c and cytochrome c peroxidase'''
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===Solvent isotope effects on interfacial protein electron transfer between cytochrome c and cytochrome c peroxidase===
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==Overview==
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D(2)O-grown crystals of yeast zinc porphyrin substituted cytochrome c peroxidase (ZnCcP) in complex with yeast iso-1-cytochrome c (yCc) diffract to higher resolution (1.7 A) and pack differently than H(2)O-grown crystals (2.4-3.0 A). Two ZnCcP's bind the same yCc (porphyrin-to-porphyrin separations of 19 and 29 A), with one ZnCcP interacting through the same interface found in the H(2)O crystals. The triplet excited-state of at least one of the two unique ZnCcP's is quenched by electron transfer (ET) to Fe(III)yCc (k(e) = 220 s(-1)). Measurement of thermal recombination ET between Fe(II)yCc and ZnCcP+ in the D(2)O-treated crystals has both slow and fast components that differ by 2 orders of magnitude (k(eb)(1) = 2200 s(-1), k(eb)(2) = 30 s(-1)). Back ET in H(2)O-grown crystals is too fast for observation, but soaking H(2)O-grown crystals in D(2)O for hours generates slower back ET, with kinetics similar to those of the D(2)O-grown crystals (k(eb)(1) = 7000 s(-1), k(eb)(2) = 100 s(-1)). Protein-film voltammetry of yCc adsorbed to mixed alkanethiol monolayers on gold electrodes shows slower ET for D(2)O-grown yCc films than for H(2)O-grown films (k(H) = 800 s(-1); k(D) = 540 s(-1) at 20 degrees C). Soaking H(2)O- or D(2)O-grown films in the counter solvent produces an immediate inverse isotope effect that diminishes over hours until the ET rate reaches that found in the counter solvent. Thus, D(2)O substitution perturbs interactions and ET between yCc and either CcP or electrode films. The effects derive from slow exchanging protons or solvent molecules that in the crystal produce only small structural changes.
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(as it appears on PubMed at http://www.pubmed.gov), where 16478190 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16478190}}
==About this Structure==
==About this Structure==
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[[Category: Kang, S A.]]
[[Category: Kang, S A.]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:06:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:29:17 2008''

Revision as of 11:29, 28 July 2008

Image:2bcn.png

Template:STRUCTURE 2bcn

Solvent isotope effects on interfacial protein electron transfer between cytochrome c and cytochrome c peroxidase

Template:ABSTRACT PUBMED 16478190

About this Structure

2BCN is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Solvent isotope effects on interfacial protein electron transfer in crystals and electrode films., Kang SA, Hoke KR, Crane BR, J Am Chem Soc. 2006 Feb 22;128(7):2346-55. PMID:16478190

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