2fte

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2fte.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2fte.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2fte| PDB=2fte | SCENE= }}
{{STRUCTURE_2fte| PDB=2fte | SCENE= }}
-
'''Bacteriophage HK97 Expansion Intermediate IV'''
+
===Bacteriophage HK97 Expansion Intermediate IV===
-
==Overview==
+
<!--
-
Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17098191}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17098191 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17098191}}
==About this Structure==
==About this Structure==
Line 20: Line 24:
==Reference==
==Reference==
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191]
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191]
 +
 +
Control of crosslinking by quaternary structure changes during bacteriophage HK97 maturation., Gan L, Conway JF, Firek BA, Cheng N, Hendrix RW, Steven AC, Johnson JE, Duda RL, Mol Cell. 2004 Jun 4;14(5):559-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15175152 15175152]
[[Category: Enterobacteria phage hk97]]
[[Category: Enterobacteria phage hk97]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 36: Line 42:
[[Category: Expansion intermediate]]
[[Category: Expansion intermediate]]
[[Category: Hk97]]
[[Category: Hk97]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:17:31 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:33:26 2008''

Revision as of 11:33, 28 July 2008

Image:2fte.png

Template:STRUCTURE 2fte

Bacteriophage HK97 Expansion Intermediate IV

Template:ABSTRACT PUBMED 17098191

About this Structure

2FTE is a Single protein structure of sequence from Enterobacteria phage hk97. Full crystallographic information is available from OCA.

Reference

Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:17098191

Control of crosslinking by quaternary structure changes during bacteriophage HK97 maturation., Gan L, Conway JF, Firek BA, Cheng N, Hendrix RW, Steven AC, Johnson JE, Duda RL, Mol Cell. 2004 Jun 4;14(5):559-69. PMID:15175152

Page seeded by OCA on Mon Jul 28 14:33:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fte"
Personal tools