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| - | [[Image:2eet.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2eet.png|left|200px]] |
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| | {{STRUCTURE_2eet| PDB=2eet | SCENE= }} | | {{STRUCTURE_2eet| PDB=2eet | SCENE= }} |
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| - | '''Guanine Riboswitch A21G, U75C mutant bound to hypoxanthine'''
| + | ===Guanine Riboswitch A21G, U75C mutant bound to hypoxanthine=== |
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| - | ==Overview==
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| - | The purine riboswitch is one of a number of mRNA elements commonly found in the 5'-untranslated region capable of controlling expression in a cis-fashion via its ability to directly bind small-molecule metabolites. Extensive biochemical and structural analysis of the nucleobase-binding domain of the riboswitch, referred to as the aptamer domain, has revealed that the mRNA recognizes its cognate ligand using an intricately folded three-way junction motif that completely encapsulates the ligand. High-affinity binding of the purine nucleobase is facilitated by a distal loop-loop interaction that is conserved between both the adenine and guanine riboswitches. To understand the contribution of conserved nucleotides in both the three-way junction and the loop-loop interaction of this RNA, we performed a detailed mutagenic survey of these elements in the context of an adenine-responsive variant of the xpt-pbuX guanine riboswitch from Bacillus subtilis. The varying ability of these mutants to bind ligand as measured by isothermal titration calorimetry uncovered the conserved nucleotides whose identity is required for purine binding. Crystallographic analysis of the bound form of five mutants and chemical probing of their free state demonstrate that the identity of several universally conserved nucleotides is not essential for formation of the RNA-ligand complex but rather for maintaining a binding-competent form of the free RNA. These data show that conservation patterns in riboswitches arise from a combination of formation of the ligand-bound complex, promoting an open form of the free RNA, and participating in the secondary structural switch with the expression platform. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17960911}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17960911 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17960911}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Rna-ligand complex]] | | [[Category: Rna-ligand complex]] |
| | [[Category: Three-way junction]] | | [[Category: Three-way junction]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:26:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:40:48 2008'' |
Revision as of 11:40, 28 July 2008
Template:STRUCTURE 2eet
Guanine Riboswitch A21G, U75C mutant bound to hypoxanthine
Template:ABSTRACT PUBMED 17960911
About this Structure
2EET is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Mutational analysis of the purine riboswitch aptamer domain., Gilbert SD, Love CE, Edwards AL, Batey RT, Biochemistry. 2007 Nov 20;46(46):13297-309. Epub 2007 Oct 26. PMID:17960911
Page seeded by OCA on Mon Jul 28 14:40:48 2008
