1t0o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1t0o.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1t0o.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1t0o| PDB=1t0o | SCENE= }}
{{STRUCTURE_1t0o| PDB=1t0o | SCENE= }}
-
'''The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose'''
+
===The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose===
-
==Overview==
+
<!--
-
The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15136043}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15136043 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15136043}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Beta-d-galactose]]
[[Category: Beta-d-galactose]]
[[Category: Glycoprotein,complex]]
[[Category: Glycoprotein,complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:21:36 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:41:58 2008''

Revision as of 11:42, 28 July 2008

Image:1t0o.png

Template:STRUCTURE 1t0o

The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose

Template:ABSTRACT PUBMED 15136043

About this Structure

1T0O is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043

Page seeded by OCA on Mon Jul 28 14:41:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t0o"
Personal tools