2frj

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{{STRUCTURE_2frj| PDB=2frj | SCENE= }}
{{STRUCTURE_2frj| PDB=2frj | SCENE= }}
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'''Nitrosyl Horse Heart Myoglobin, Nitrite/Dithionite Method'''
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===Nitrosyl Horse Heart Myoglobin, Nitrite/Dithionite Method===
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==Overview==
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Nitrite is an important species in the global nitrogen cycle, and the nitrite reductase enzymes convert nitrite to nitric oxide (NO). Recently, it has been shown that hemoglobin and myoglobin catalyze the reduction of nitrite to NO under hypoxic conditions. We have determined the 1.20 A resolution crystal structure of the nitrite adduct of ferric horse heart myoglobin (hh Mb). The ligand is bound to iron in the nitrito form, and the complex is formulated as MbIII(ONO-). The Fe-ONO bond length is 1.94 A, and the O-N-O angle is 113 degrees . In addition, the nitrite ligand is stabilized by hydrogen bonding with the distal His64 residue. We have also determined the 1.30 A resolution crystal structures of hh MbIINO. When hh MbIINO is prepared from the reaction of metMbIII with nitrite/dithionite, the FeNO angle is 144 degrees with a Fe-NO bond length of 1.87 A. However, when prepared from the reaction of NO with reduced MbII, the FeNO angle is 120 degrees with a Fe-NO bond length of 2.13 A. This difference in FeNO conformations as a function of preparative method is reproducible, and suggests a role of the distal pocket in hh MbIINO in stabilizing local FeNO conformational minima.
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(as it appears on PubMed at http://www.pubmed.gov), where 16777231 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16777231}}
==About this Structure==
==About this Structure==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:00:35 2008''

Revision as of 12:00, 28 July 2008

Image:2frj.png

Template:STRUCTURE 2frj

Nitrosyl Horse Heart Myoglobin, Nitrite/Dithionite Method

Template:ABSTRACT PUBMED 16777231

About this Structure

2FRJ is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

Reference

Crystal structures of the nitrite and nitric oxide complexes of horse heart myoglobin., Copeland DM, Soares AS, West AH, Richter-Addo GB, J Inorg Biochem. 2006 Aug;100(8):1413-25. Epub 2006 May 5. PMID:16777231

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