1sau

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[[Image:1sau.gif|left|200px]]
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{{STRUCTURE_1sau| PDB=1sau | SCENE= }}
{{STRUCTURE_1sau| PDB=1sau | SCENE= }}
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'''The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution'''
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===The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution===
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==Overview==
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The structure of the 115 amino-acid residue protein DsvC was determined based on the anomalous scattering provided by the five S atoms present in the structure. By collecting the diffraction data at a wavelength of 1.9 A, the anomalous signal provided by the S atoms was enhanced. However, significant radiation damage occurred during the course of the experiment, which led to differences between different parts of the data set. Only by dividing the total data set into five data sets was it possible to obtain phases; these could then be successfully extended to allow structure determination by the automated model-building program ARP/wARP. A computational correction for the radiation damage was found to significantly improve the success rate in determining the heavy-atom substructure and to improve phasing and refinement statistics.
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The line below this paragraph, {{ABSTRACT_PUBMED_15039557}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15039557 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15039557}}
==About this Structure==
==About this Structure==
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[[Category: Weiss, M S.]]
[[Category: Weiss, M S.]]
[[Category: Orthogonal helical bundle]]
[[Category: Orthogonal helical bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:29:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:02:16 2008''

Revision as of 12:02, 28 July 2008

Image:1sau.png

Template:STRUCTURE 1sau

The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution

Template:ABSTRACT PUBMED 15039557

About this Structure

1SAU is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.

Reference

Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing., Weiss MS, Mander G, Hedderich R, Diederichs K, Ermler U, Warkentin E, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):686-95. Epub 2004, Mar 23. PMID:15039557

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