2rmk

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{{STRUCTURE_2rmk| PDB=2rmk | SCENE= }}
{{STRUCTURE_2rmk| PDB=2rmk | SCENE= }}
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'''Rac1/PRK1 Complex'''
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===Rac1/PRK1 Complex===
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==Overview==
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Protein kinase C-related kinase 1 (PRK1 or PKN) is involved in regulation of the intermediate filaments of the actin cytoskeleton, as well as having effects on processes as diverse as mitotic timing and apoptosis. It is activated by interacting with the Rho family small G proteins and arachidonic acid or by caspase cleavage. We have previously shown that the HR1b of PRK1 binds exclusively to Rac1, whereas the HR1a domain binds to both Rac1 and RhoA. Here, we have determined the solution structure of the HR1b-Rac complex. We show that HR1b binds to the C-terminal end of the effector loop and switch 2 of Rac1. Comparison with the HR1a-RhoA structure shows that this part of the Rac1-HR1b interaction is homologous to one of the contact sites that HR1a makes with RhoA. The Rac1 used in this study included the C-terminal polybasic region, which is frequently omitted from structural studies, as well as the core G domain. The Rac1 C-terminal region reverses in direction to interact with residues in switch 2, and the polybasic region itself interacts with residues in HR1b. The interactions with HR1b do not prevent the polybasic region being available to contact the negatively charged membrane phospholipids, which is considered to be its primary role. This is the first structural demonstration that the C terminus of a G protein forms a novel recognition element for effector binding.
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{{ABSTRACT_PUBMED_18006505}}
==About this Structure==
==About this Structure==
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2RMK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RMK OCA].
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2RMK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RMK OCA].
==Reference==
==Reference==
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[[Category: Serine/threonine-protein kinase]]
[[Category: Serine/threonine-protein kinase]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:10:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:02:29 2008''

Revision as of 12:02, 28 July 2008

Image:2rmk.png

Template:STRUCTURE 2rmk

Rac1/PRK1 Complex

Template:ABSTRACT PUBMED 18006505

About this Structure

2RMK is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

The Rac1 polybasic region is required for interaction with its effector PRK1., Modha R, Campbell LJ, Nietlispach D, Buhecha HR, Owen D, Mott HR, J Biol Chem. 2008 Jan 18;283(3):1492-500. Epub 2007 Nov 15. PMID:18006505

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