2van

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{{STRUCTURE_2van| PDB=2van | SCENE= }}
{{STRUCTURE_2van| PDB=2van | SCENE= }}
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'''NUCLEOTIDYL TRANSFER MECHANISM OF MISMATCHED DNTP INCORPORATION BY DNA POLYMERASE B BY STRUCTURAL AND KINETIC ANALYSES'''
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===NUCLEOTIDYL TRANSFER MECHANISM OF MISMATCHED DNTP INCORPORATION BY DNA POLYMERASE B BY STRUCTURAL AND KINETIC ANALYSES===
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==Overview==
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Understanding how DNA polymerases control fidelity requires elucidation of the mechanisms of matched and mismatched dNTP incorporations. Little is known about the latter because mismatched complexes do not crystallize readily. In this report, we employed small-angle X-ray scattering (SAXS) and structural modeling to probe the conformations of different intermediate states of mammalian DNA polymerase beta (Pol beta) in its wild-type and an error-prone variant, I260Q. Our structural results indicate that the mismatched ternary complex lies in-between the open and the closed forms, but more closely resembles the open form for WT and the closed form for I260Q. On the basis of molecular modeling, this over-stabilization of mismatched ternary complex of I260Q is likely caused by formation of a hydrogen bonding network between the side chains of Gln(260), Tyr(296), Glu(295) and Arg(258), freeing up Asp(192) to coordinate MgdNTP. These results argue against recent reports suggesting that mismatched dNTP incorporations follow a conformational path distinctly different from that of matched dNTP incorporation, or that its conformational closing is a major contributor to fidelity.
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{{ABSTRACT_PUBMED_18385153}}
==About this Structure==
==About this Structure==
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[[Category: Sodium]]
[[Category: Sodium]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 12:03, 28 July 2008

Image:2van.png

Template:STRUCTURE 2van

NUCLEOTIDYL TRANSFER MECHANISM OF MISMATCHED DNTP INCORPORATION BY DNA POLYMERASE B BY STRUCTURAL AND KINETIC ANALYSES

Template:ABSTRACT PUBMED 18385153

About this Structure

2VAN is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Mismatched dNTP incorporation by DNA polymerase {beta} does not proceed via globally different conformational pathways., Tang KH, Niebuhr M, Tung CS, Chan HC, Chou CC, Tsai MD, Nucleic Acids Res. 2008 Apr 2;. PMID:18385153

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