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1xzq

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{{STRUCTURE_1xzq| PDB=1xzq | SCENE= }}
{{STRUCTURE_1xzq| PDB=1xzq | SCENE= }}
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'''Structure of the GTP-binding protein TrmE from Thermotoga maritima complexed with 5-formyl-THF'''
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===Structure of the GTP-binding protein TrmE from Thermotoga maritima complexed with 5-formyl-THF===
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==Overview==
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TrmE is a 50 kDa guanine nucleotide-binding protein conserved between bacteria and man. It is involved in the modification of uridine bases (U34) at the first anticodon (wobble) position of tRNAs decoding two-family box triplets. The precise role of TrmE in the modification reaction is hitherto unknown. Here, we report the X-ray structure of TrmE from Thermotoga maritima. The structure reveals a three-domain protein comprising the N-terminal alpha/beta domain, the central helical domain and the G domain, responsible for GTP binding and hydrolysis. The N-terminal domain induces dimerization and is homologous to the tetrahydrofolate-binding domain of N,N-dimethylglycine oxidase. Biochemical and structural studies show that TrmE indeed binds formyl-tetrahydrofolate. A cysteine residue, necessary for modification of U34, is located close to the C1-group donor 5-formyl-tetrahydrofolate, suggesting a direct role of TrmE in the modification analogous to DNA modification enzymes. We propose a reaction mechanism whereby TrmE actively participates in the formylation reaction of uridine and regulates the ensuing hydrogenation reaction of a Schiff's base intermediate.
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{{ABSTRACT_PUBMED_15616586}}
==About this Structure==
==About this Structure==
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[[Category: Thf-binding]]
[[Category: Thf-binding]]
[[Category: Trna-modification]]
[[Category: Trna-modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:42:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:03:53 2008''

Revision as of 12:03, 28 July 2008

Image:1xzq.png

Template:STRUCTURE 1xzq

Structure of the GTP-binding protein TrmE from Thermotoga maritima complexed with 5-formyl-THF

Template:ABSTRACT PUBMED 15616586

About this Structure

1XZQ is a Protein complex structure of sequences from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

The structure of the TrmE GTP-binding protein and its implications for tRNA modification., Scrima A, Vetter IR, Armengod ME, Wittinghofer A, EMBO J. 2005 Jan 12;24(1):23-33. Epub 2004 Dec 16. PMID:15616586

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